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NME1 Antibody (711457) in ICC/IF
For immunofluorescence analysis, SK-OV-3 cells were fixed and permeabilized for detection of endogenous NME1 using Anti- NME1 Recombinant Rabbit Superclonal™ Antibody (Product # 711457, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NME1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36... View More
Product Details
711457
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
11HCLC
Immunogen
Protein corresponding to Human NME1 (1-152)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633181
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Pig and Bovine
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: expressed in non-metastatic cells 1 protein; expressed in non-metastatic cells 1, protein; GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor NM23; NDK A; NDP kinase A; NDPK-A; NM23-H1; nm23-M1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; Tumor metastatic process-associated protein
Gene Aliases: AL024257; AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NM23-M1; NM23A; NME1
UniProt ID: (Human) P15531, (Mouse) P15532
Entrez Gene ID: (Human) 4830, (Mouse) 18102
magnesium ion binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein binding
ATP binding
GTP binding
intermediate filament binding
protein kinase binding
identical protein binding
gamma-tubulin binding
ribosomal small subunit binding
poly(A) RNA binding
nucleotide binding
kinase activity
transferase activity
enzyme binding
metal ion binding
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
DNA metabolic process
endocytosis
lactation
negative regulation of cell proliferation
nucleoside triphosphate biosynthetic process
negative regulation of gene expression
positive regulation of neuron projection development
response to amine
nucleobase-containing small molecule interconversion
hippocampus development
response to testosterone
cellular response to drug
regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
response to cAMP
cellular response to glucose stimulus
cellular response to fatty acid
nervous system development
nucleotide metabolic process
phosphorylation
cell differentiation
mammary gland development
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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