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Invitrogen
NME2 Polyclonal Antibody
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FIGURE: 1 / 7
NME2 Antibody (PA5-96679) in ICC/IF
Immunofluorescence analysis of Nucleoside diphosphate kinase B was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NME2 Polyclonal Antibody (Product # PA5-96679) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488... View More
Product Details
PA5-96679
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human NME2/NM23B (NP_0010181481)
Conjugate
Form
Liquid
Concentration
0.31 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808481
Product Specific Information
Immunogen sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE; Positive Samples: PC-12, 22Rv1, Mouse kidney, Mouse liver, Rat liver; Cellular Location: Cell projection, Cytoplasm, Nucleus, lamellipodium, ruffle
Target Information
Nucleoside diphosphate kinase B is an enzyme that in humans is encoded by the NME2 gene. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein consisting of sequence sharing identity with each individual gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2.7.13.3; C-myc purine-binding transcription factor PUF; c-myc transcription factor; epididymis secretory sperm binding protein Li 155an; expressed in non-metastatic cells 2; expressed in non-metastatic cells 2 protein; HEL-S-155an; Histidine protein kinase NDKB; NDK B; NDP kinase alpha; NDP kinase B; nm23-H2; nm23-M2; non-metastatic cells 2, protein (NM23) expressed in; non-metastatic cells 2, protein (NM23B) expressed in; Nucleoside diphosphate kinase B; nucleoside-diphosphate kinase 2; nucleotide diphosphate kinase B; P18
Gene Aliases: NDKB; NDPK-B; NDPKB; nm23-2; NM23-H2; nm23-M2; NM23B; NME2; p18-12d; PUF
UniProt ID: (Human) P22392, (Rat) P19804, (Mouse) Q01768
Entrez Gene ID: (Human) 4831, (Rat) 83782, (Mouse) 18103
DNA binding
transcription factor activity, sequence-specific DNA binding
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein binding
ATP binding
metal ion binding
protein serine/threonine kinase activity
fatty acid binding
drug binding
intermediate filament binding
enzyme binding
nucleotide binding
kinase activity
transferase activity
negative regulation of myeloid leukocyte differentiation
nucleoside diphosphate phosphorylation
GTP biosynthetic process
UTP biosynthetic process
CTP biosynthetic process
integrin-mediated signaling pathway
nucleoside triphosphate biosynthetic process
positive regulation of neuron projection development
peptidyl-histidine phosphorylation
positive regulation of cAMP biosynthetic process
cellular response to oxidative stress
regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of keratinocyte differentiation
regulation of epidermis development
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
positive regulation of epithelial cell proliferation
protein oligomerization
response to growth hormone
cellular response to glucose stimulus
cellular response to fatty acid
purine nucleotide metabolic process
pyrimidine nucleotide metabolic process
nucleotide metabolic process
phosphorylation
mitophagy in response to mitochondrial depolarization
transcription, DNA-templated
regulation of transcription, DNA-templated
cell adhesion
nucleobase-containing small molecule interconversion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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