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Invitrogen
NMI Polyclonal Antibody
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FIGURE: 1 / 9
NMI Antibody (PA5-79745) in ICC/IF
Immunocytochemistry analysis of NMI using anti-NMI antibody (Product # PA5-79745) . NMI was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NMI antibody (Product # PA5-79745) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More
Product Details
PA5-79745
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NMI recombinant protein (Position: E2-E307).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746860
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human K562 whole cell, human Caco-2 whole cell, human A431 whole cell, human PC-3 whole cell, human 293T whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human mammary cancer tissue, human mammary cancer tissue, human placenta tissue, human placenta tissue. ICC/IF: A431 cell. Flow: U937 cell.
Target Information
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N-myc (and STAT) interactor; N-myc and STAT interactor; N-myc interactor; N-myc-interactor; Nmi
Gene Aliases: NMI
UniProt ID: (Human) Q13287
Entrez Gene ID: (Human) 9111
transcription from RNA polymerase II promoter
inflammatory response
JAK-STAT cascade
negative regulation of interferon-alpha biosynthetic process
negative regulation of interferon-beta biosynthetic process
negative regulation of innate immune response
interferon-gamma-mediated signaling pathway
positive regulation of protein K48-linked ubiquitination
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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