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Invitrogen
NMT1 Polyclonal Antibody
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FIGURE: 1 / 5
NMT1 Antibody (PA5-78082) in ICC/IF
NMT1 Polyclonal Antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMT1 protein stained by NMT1 Polyclonal Antibody (Product # PA5-78082) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78082
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736241
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NMT1 is involved with Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1 MIM 139311).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternative, short form NMT-S; Glycylpeptide N-tetradecanoyltransferase 1; HsNMT1; long form, NMT-L; Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; type I N-myristoyltransferase
Gene Aliases: AW536594; NMT; NMT1
UniProt ID: (Human) P30419, (Mouse) O70310, (Rat) Q8K1Q0
Entrez Gene ID: (Human) 4836, (Mouse) 18107, (Rat) 259274
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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