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NMU Antibody (PA5-79746) in WB
Western blot analysis of NMU in Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NMU polyclonal antibody (Product # PA5-79746) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat a... View More
Product Details
PA5-79746
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human NMU (FRVDEEFQSPFASQSRGYFLFRPRN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746861
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, mouse kidney tissue.
Target Information
This gene encodes a member of the neuromedin family of neuropeptides. The encoded protein is a precursor that is proteolytically processed to generate a biologically active neuropeptide that plays a role in pain, stress, immune-mediated inflammatory diseases and feeding regulation. Increased expression of this gene was observed in renal, pancreatic and lung cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. Some of these isoforms may undergo similar processing to generate the mature peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neuromedin-U; NmU 25; prepro-NMU
Gene Aliases: NMU
UniProt ID: (Human) P48645, (Rat) P12760, (Mouse) Q9QXK8
Entrez Gene ID: (Human) 10874, (Rat) 63887, (Mouse) 56183
gastric acid secretion
neuropeptide signaling pathway
photoperiodism
sensory perception of pain
eating behavior
positive regulation of smooth muscle contraction
positive regulation of hormone secretion
positive regulation of synaptic transmission
regulation of smooth muscle contraction
signal transduction
G-protein coupled receptor signaling pathway
digestion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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