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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NNT Antibodies

          Invitrogen

          NNT Monoclonal Antibody (8B4BB10)

          1 Reference
          View all (11) NNT antibodies

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          Cite NNT Monoclonal Antibody (8B4BB10)

          • Antibody Testing Data (6)
          NNT Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NNT Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          NNT Antibody (459170) in ICC/IF

          Immunofluorescent analysis of NNT in Human skin fibroblasts using an NNT Monoclonal antibody (Product # 459170). Cultured, normal Human skin fibroblasts (strain HDFn) were labeled with an NNT Monoclonal antibody (Product # 459170) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          NNT Antibody in Immunocytochemistry (ICC/IF)
          NNT Antibody in Immunohistochemistry (IHC)
          NNT Antibody in Western Blot (WB)
          NNT Antibody in Western Blot (WB)
          NNT Antibody in Western Blot (WB)
          NNT Antibody in Flow Cytometry (Flow)

          Product Details

          459170

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          1:100
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1 µg/mL
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          8B4BB10

          Immunogen

          Human heart mitochondria
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Ammonium sulfate precipitation

          Storage buffer

          HEPES buffered saline

          Contains

          0.02% sodium azide

          Storage conditions

          4°C, do not freeze

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10375040

          Product Specific Information

          Positive control: Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.

          Target Information

          This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: NAD(P) transhydrogenase, mitochondrial; Nicotinamide nucleotide transhydrogenase; Nicotinamide nucleotide transhydrogenase (NAD(P)+ transhydrogenase); Pyridine nucleotide transhydrogenase

          View more View less

          Gene Aliases: 4930423F13Rik; AI323702; BB168308; BOS_19786; GCCD4; NNT

          View more View less

          UniProt ID: (Bovine) P11024, (Human) Q13423

          View more View less

          Entrez Gene ID: (Bovine) 280878, (Human) 23530, (Rat) 310378, (Mouse) 18115

          View more View less

          Function(s)
          NAD(P)+ transhydrogenase (AB-specific) activity NADP binding NAD(P)+ transhydrogenase (B-specific) activity NAD(P)+ transhydrogenase activity NAD binding oxidoreductase activity
          Process(es)
          proton transport reactive oxygen species metabolic process cell redox homeostasis oxidation-reduction process tricarboxylic acid cycle NADPH regeneration hydrogen ion transmembrane transport
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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