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Invitrogen
NO66 Polyclonal Antibody
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Product Details
PA5-31439
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human C14orf169/NO66. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548913
Product Specific Information
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L8 histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; FLJ21802; histone lysine demethylase NO66; hsNO66; lysine-specific demethylase NO66; MYC-associated protein with JmjC domain; Nucleolar protein 66; Ribosomal oxygenase 1; Ribosomal oxygenase NO66; ROX; up-regulated in lung cancer 2
Gene Aliases: C14orf169; hsNO66; MAPJD; NO66; RIOX1; ROX; URLC2
UniProt ID: (Human) Q9H6W3
Entrez Gene ID: (Human) 79697
iron ion binding
protein binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K36 specific)
chromatin remodeling
transcription, DNA-templated
peptidyl-amino acid modification
histone H3-K4 demethylation
ribosome biogenesis
post-translational protein modification
negative regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
oxidation-reduction process
histone H3-K36 demethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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