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NOD3 Antibody (PA5-115658) in ICC/IF
Immunocytochemistry analysis of NOD3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115658) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NLRC3(Accession Q7RTR2), corresponding to amino acid residues L1026-M1065.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900293
Product Specific Information
Antibody detects endogenous levels of total NLRC3.
Target Information
NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-βB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARD15-like protein; caterpillar 16.2; Caterpiller 16.2; Caterpiller protein 16.2; CLR16.2; NACHT, LRR and CARD domains-containing protein 3; NLR family CARD domain-containing protein 3; NLR family, CARD domain containing 3; NLR family, CARD domain containing 3, pseudogene 1; NOD-like receptor C3; Nucleotide-binding oligomerization domain protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3; Protein NLRC3
Gene Aliases: CLR16.2; D230007K08Rik; mFLJ00348; NLRC3; Nlrc3-ps1; NOD3; RGD1304902
UniProt ID: (Human) Q7RTR2, (Mouse) Q5DU56
Entrez Gene ID: (Human) 197358, (Rat) 287070, (Mouse) 268857
I-kappaB kinase/NF-kappaB signaling
negative regulation of NF-kappaB transcription factor activity
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
T cell activation
negative regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein ubiquitination
response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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