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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • NOLC1 Antibodies

          Invitrogen

          NOLC1 Polyclonal Antibody

          View all (17) NOLC1 antibodies
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          Datasheet
          Protocols
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          Cite NOLC1 Polyclonal Antibody

          • Antibody Testing Data (3)
          NOLC1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NOLC1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          NOLC1 Antibody (PA5-35938) in ICC/IF

          NOLC1 Polyclonal Antibody detects NOLC1 protein at cytoplasm and nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NOLC1 protein stained by NOLC1 Polyclonal Antibody (Product # PA5-35938) diluted at 1:500. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          NOLC1 Antibody in Immunocytochemistry (ICC/IF)
          NOLC1 Antibody in Western Blot (WB)
          NOLC1 Antibody in Western Blot (WB)

          Product Details

          PA5-35938

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the C-terminus region of human NOLC1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2553248

          Product Specific Information

          Recommended positive controls: H1299, HCT116, K562, HL-60.

          Predicted reactivity: Bovine (82%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 140 kDa nucleolar phosphoprotein; HCV NS5A trans-regulated protein 13; HCV NS5A-transactivated protein 13; Hepatitis C virus NS5A-transactivated protein 13; Nopp140; Nucleolar 130 kDa protein; Nucleolar and coiled-body phosphoprotein 1; nucleolar and coiled-body phosphprotein 1; Nucleolar phosphoprotein p130; nucleolar protein p130

          View more View less

          Gene Aliases: KIAA0035; NOLC1; NOPP130; NOPP140; NS5ATP13; P130

          View more View less

          UniProt ID: (Human) Q14978

          View more View less

          Entrez Gene ID: (Human) 9221

          View more View less

          Function(s)
          protein binding ATP binding GTP binding poly(A) RNA binding
          Process(es)
          rRNA processing nucleolus organization cell cycle mitotic nuclear division
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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