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Invitrogen
NONO Polyclonal Antibody, Biotin
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NONO Antibody (PA5-142222) in WB
Western Blot analysis of NONO in Mouse Brain lysate (35 µg protein in RIPA buffer) using NONO Polyclonal Antibody, Biotin (Product # PA5-142222) at 0.1 µg/mL incubated for 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Product Details
PA5-142222
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-NRAAPGAEFAPNK (aa 455-467).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932876
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:7871; NMT-1; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing; NonO protein
Gene Aliases: AA407051; AV149256; nonA; Nono; NRB54; P54NRB
UniProt ID: (Mouse) Q99K48
Entrez Gene ID: (Mouse) 53610
DNA repair
DNA recombination
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA processing
cellular response to DNA damage stimulus
circadian rhythm
RNA splicing
regulation of circadian rhythm
negative regulation of transcription, DNA-templated
rhythmic process
negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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