Invitrogen

NONO Monoclonal Antibody (78-1 C)

NONO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NONO Antibody (MA3-2024) in IHC (P)

Immunohistochemistry was performed on cancer biopsies of deparaffinized human Bladder carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse mon... View More

NONO Antibody in Immunoprecipitation (IP)

NONO Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

NONO Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Non-POU domain-containing octamer-binding protein siRNA and decrease in signal intensity was observed in Western Blot application using Anti-NONO Monoclonal Antibody (78-1 C) (Product # MA3-2024). {KD}

Product Details

MA3-2024

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

78-1 C

Immunogen

Synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2152170

Product Specific Information

MA3-2024 detects the nmt55/p54nrb in human, rat and rabbit samples.

MA3-2024 has been successfully used in immunohistochemistry, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~55 kDa protein representing nmt55/p54nrb in MCF-7 cells, human breast tumor cells, rat uterine tissue, and rabbit liver extracts. In immunohistochemistry this antibody recognizes the nmt55/p54nrb in MCF-7 cells and human breast tumor cells. This monoclonal antibody has been referred to as
NMT-1.

The MA3-2024 immunogen is a synthetic peptide corresponding to residues T(140) V R E A G P P A F Y R P N S(154) of human ER alpha.

Target Information

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AATF/Che-1 localizes to paraspeckles and suppresses R-loops accumulation and interferon activation in Multiple Myeloma.

The EMBO journal

Bruno T,Corleone G,Catena V,Cortile C,De Nicola F,Fabretti F,Gumenyuk S,Pisani F,Mengarelli A,Passananti C,Fanciulli M

MA3-2024 was used in Immunoprecipitation to indicate that Che-1/NEAT1 cooperation prevents excessive inflammatory signaling in Multiple Myeloma by facilitating the clearance of R-loops.

Thu Nov 17 00:00:00 UTC 2022

TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.

Nucleic acids research

Yarosh CA,Tapescu I,Thompson MG,Qiu J,Mallory MJ,Fu XD,Lynch KW

MA3-2024 was used in western blot to investigate the interaction between TRAP150 and PSF and its effect in T cells

Thu Oct 15 00:00:00 UTC 2015

Role of NonO-histone interaction in TNFalpha-suppressed prolyl-4-hydroxylase alpha1.

Biochimica et biophysica acta

Zhang C,Zhang MX,Shen YH,Burks JK,Li XN,LeMaire SA,Yoshimura K,Aoki H,Matsuzaki M,An FS,Engler DA,Matsunami RK,Coselli JS,Zhang Y,Wang XL

MA3-2024 was used in immunoprecipitation and western blot to investigate the effect of TNFalpha on prolyl-4 hydroxylase alpha1

Fri Aug 01 00:00:00 UTC 2008

Role of NonO-histone interaction in TNFalpha-suppressed prolyl-4-hydroxylase alpha1.

Biochimica et biophysica acta

Zhang C,Zhang MX,Shen YH,Burks JK,Li XN,LeMaire SA,Yoshimura K,Aoki H,Matsuzaki M,An FS,Engler DA,Matsunami RK,Coselli JS,Zhang Y,Wang XL

MA3-2024 was used in immunoprecipitation and western blot to investigate the effect of TNFalpha on prolyl-4 hydroxylase alpha1

Fri Aug 01 00:00:00 UTC 2008

Combinatorial control of signal-induced exon repression by hnRNP L and PSF.

Molecular and cellular biology

Melton AA,Jackson J,Wang J,Lynch KW

MA3-2024 was used in western blot to investigate the mechanism for signal-regulated alternative splicing

Mon Oct 01 00:00:00 UTC 2007

p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation.

RNA (New York, N.Y.)

Liang S,Lutz CS

MA3-2024 was used in western blot to investigate the roles of the p54nrb during the pre-mRNA cleavage.

Sun Jan 01 00:00:00 UTC 2006

Multisite phosphorylation of Pin1-associated mitotic phosphoproteins revealed by monoclonal antibodies MPM-2 and CC-3.

BMC cell biology

Albert AL,Lavoie SB,Vincent M

MA3-2024 was used in western blot to investigate the phosphorylation pattern of mitotic phosphoprotein

Tue Jun 01 00:00:00 UTC 2004

Immunodetection of nmt55/p54nrb isoforms in human breast cancer.

BMC cancer

Pavao M,Huang YH,Hafer LJ,Moreland RB,Traish AM

MA3-2024 was used in immunohistochemistry to investigate the expression level of nmt55/p54nrb isoforms in human breast cancer

Wed Nov 13 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; HGNC:7871; NMT-1; NMT55; non-POU domain containing, octamer-binding-like; non-POU domain-containing octamer (ATGCAAAT) binding protein; Non-POU domain-containing octamer-binding protein; non-POU-domain-containing; non-POU-domain-containing, octamer-binding protein; NonO protein; p54(nrb); protein phosphatase 1, regulatory subunit 114; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)-like

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Gene Aliases: MRXS34; NMT55; NONO; NRB54; P54; P54NRB; PPP1R114

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UniProt ID: (Human) Q15233, (Rat) Q5FVM4

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Entrez Gene ID: (Rabbit) 100346936, (Rabbit) 100348685, (Human) 4841, (Rat) 317259

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Function(s)

nucleotide binding transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding chromatin binding protein binding identical protein binding poly(A) RNA binding

Process(es)

mRNA splicing, via spliceosome DNA repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing circadian rhythm RNA splicing regulation of circadian rhythm negative regulation of transcription, DNA-templated negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NONO Monoclonal Antibody (78-1 C)

NONO Antibody (MA3-2024) in IHC (P)

Product Details

MA3-2024

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

ChIP assay (ChIP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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