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Invitrogen
NOP14 Polyclonal Antibody
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FIGURE: 1 / 1
NOP14 Antibody (PA5-87842) in WB
Western blot analysis of NOP14 in extracts of various cell lines. Samples were incubated with NOP14 Polyclonal antibody (Product # PA5-87842) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-87842
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NOP14 (NP_0012789081)
Conjugate
Form
Liquid
Concentration
1.12 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804449
Product Specific Information
Immunogen sequence: MAKAKKVGAR RKASGAPAGA RGGPAKANSN PFEVKVNRQK FQILGRKTRH DVGLPGVSRA RALRKRTQTL LKEYKERDKS NVFRDKRFGE YNSNMSPEEK MMKRFALEQQ RHHEKKSIYN LNEDEELTHY GQSLADIEKH NDIVDSDSDA EDRGTLSAEL TAAHFGGGGG LLHKKTQQEG EEREKPKSRK ELIEELIAKS KQEKRERQAQ REDALELTEK LDQDWKEIQT LLSHKTPKSE; Positive Samples: HeLa, U-87MG, HT-29, Jurkat, MCF7; Cellular Location: Nucleus, nucleolus
Target Information
Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NOP14 nucleolar protein homolog; Nucleolar complex protein 14; Nucleolar protein 14; probable nucleolar complex protein 14
Gene Aliases: C4orf9; NOL14; NOP14; RES4-25; RES425; UTP2
UniProt ID: (Human) P78316
Entrez Gene ID: (Human) 8602
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
rRNA processing
maturation of SSU-rRNA
ribosomal small subunit biogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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