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Invitrogen
NOP16 Polyclonal Antibody
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FIGURE: 1 / 1
NOP16 Antibody (PA5-89637) in WB
Western blot analysis of NOP16 in extracts of various cell lines. Samples were incubated with NOP16 Polyclonal antibody (Product # PA5-89637) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-89637
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human NOP16 (NP_0574752)
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805703
Product Specific Information
Immunogen sequence: MPKAKGKTRR QKFGYSVNRK RLNRNARRKA APRIECSHIR HAWDHAKSVR QNLAEMGLAV DPNRAVPLRK RKVKAMEVDI EERPKELVRK PYVLNDLEAE ASLPEKKGNT LSRDLIDYVR YMVENHGEDY KAMARDEKNY YQDTPKQIRS KINVYKRFYP AEWQDFLDSL QKRKMEVE; Positive Samples: HT-29, A-549, A-431, MCF-7, HeLa, Mouse thymus, Rat spleen; Cellular Location: Nucleus, nucleolus
Target Information
NOP16 is transcriptionally regulated by c-Myc (MYC), upregulated in breast cancer, and overexpression is associated with poor patient survival (Butt et al., 2008).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HBV pre-S2 trans-regulated protein 3; hypothetical protein HSPC111; NOP16 nucleolar protein homolog; Nucleolar protein 16; nucleolar protein 16 homolog; Protein ZH1
Gene Aliases: AA409471; CGI-117; D13Wsu177e; HSPC111; HSPC185; NOP16; RGD1305727
UniProt ID: (Human) Q9Y3C1, (Mouse) Q9CPT5, (Rat) Q1RP77
Entrez Gene ID: (Human) 51491, (Mouse) 28126, (Rat) 306768
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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