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Rockland
NOTCH 2 Polyclonal Antibody
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FIGURE: 1 / 2
NOTCH 2 Antibody (100-401-406) in IHC (P)
Rocklands Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Product Details
100-401-406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chimpanzee,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
Conjugate
Form
Liquid
Concentration
75 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
Target Information
Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved Notch signaling pathway protein. NOTCH2 is a type 1 transmembrane protein with an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. NOTCH2 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurogenic locus notch homolog protein 2; Notch 2; Notch homolog 2
Gene Aliases: AGS2; HJCYS; hN2; NOTCH2
UniProt ID: (Human) Q04721
Entrez Gene ID: (Human) 4853, (Dog) 483148
cell fate determination
marginal zone B cell differentiation
pulmonary valve morphogenesis
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
apoptotic process
cell cycle arrest
Notch signaling pathway
multicellular organism development
nervous system development
negative regulation of cell proliferation
organ morphogenesis
cell growth
stem cell population maintenance
hemopoiesis
intracellular receptor signaling pathway
tissue regeneration
negative regulation of apoptotic process
positive regulation of Ras protein signal transduction
bone remodeling
regulation of developmental process
atrial septum morphogenesis
Notch signaling involved in heart development
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