Invitrogen

NOTCH2 Monoclonal Antibody (16F11), PE, eBioscience™

NOTCH2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

NOTCH2 Antibody (12-5786-82) in Flow

Staining of unstimulated (blue histogram) or 24-hour anti-CD3-stimulated (Product # 16-0031-82) (purple histogram) C57Bl/6 splenocytes with 0.5 µg of Anti-Mouse Notch2 PE. Cells in the lymphocyte gate were used for analysis.

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Product Details

12-5786-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

16F11

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10670497

Product Specific Information

Description: This 16F11 monoclonal antibody reacts with the extracellular domain of human and mouse Notch2, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.

Notch2 is expressed on thymocytes (i.e., DN1 and DN2 subsets), activated peripheral T cells, and bone marrow. Moreover, expression of Notch2 has been reported to be altered in various cancers and lymphomas. In addition to its role in stem cell hematopoiesis, Notch2 plays a pivotal role in marginal zone B cell development. Notch2 also interacts with phosphorylated CREB1 to induce granzyme B transcription in cytotoxic T cells.

Applications Reported: This 16F11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 16F11 antibody has been tested by flow cytometric analysis of 24-hour anti-CD3-stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved Notch signaling pathway protein. NOTCH2 is a type 1 transmembrane protein with an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. NOTCH2 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways.

Molecular cancer

Ibrahim SA,Gadalla R,El-Ghonaimy EA,Samir O,Mohamed HT,Hassan H,Greve B,El-Shinawi M,Mohamed MM,Götte M

12-5786 was used in Flow cytometry/Cell sorting to identify Syndecan-1 as a molecular marker in IBC patients, and demonstrate its role in fine-tuning IL-6/STAT3, Notch and EGFR signaling pathways.

Tue Mar 07 00:00:00 UTC 2017

NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AML.

Blood

Adamia S,Bar-Natan M,Haibe-Kains B,Pilarski PM,Bach C,Pevzner S,Calimeri T,Avet-Loiseau H,Lode L,Verselis S,Fox EA,Galinsky I,Mathews S,Dagogo-Jack I,Wadleigh M,Steensma DP,Motyckova G,Deangelo DJ,Quackenbush J,Tenen DG,Stone RM,Griffin JD

12-5786 was used in Flow cytometry/Cell sorting to suggest that NOTCH2 and FLT3 mis-splicing is a common characteristic of AML and has the potential to generate transcripts encoding proteins with altered function.

Thu May 01 00:00:00 UTC 2014

Notch pathway activation targets AML-initiating cell homeostasis and differentiation.

The Journal of experimental medicine

Lobry C,Ntziachristos P,Ndiaye-Lobry D,Oh P,Cimmino L,Zhu N,Araldi E,Hu W,Freund J,Abdel-Wahab O,Ibrahim S,Skokos D,Armstrong SA,Levine RL,Park CY,Aifantis I

12-5786 was used in Flow cytometry/Cell sorting to demonstrate a novel tumour suppressor role for Notch signalling in acute myeloid leukaemia.

Mon Feb 11 00:00:00 UTC 2013

Interleukin-19: a constituent of the regulome that controls antigen presenting cells in the lungs and airway responses to microbial products.

PloS one

Hoffman C,Park SH,Daley E,Emson C,Louten J,Sisco M,de Waal Malefyt R,Grunig G

12-5786 was used in Flow cytometry/Cell sorting to examine the role of IL-19 as a constituent of the regulome controlling MHC II antigen display and Notch2 signalling in the lungs.

Mon Mar 26 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Motch B; Neurogenic locus notch homolog protein 2; Notch 2; Notch gene homolog 2; Notch homolog 2

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Gene Aliases: AGS2; AI853703; HJCYS; hN2; N2; NOTCH2

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UniProt ID: (Human) Q04721, (Mouse) O35516

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Entrez Gene ID: (Human) 4853, (Mouse) 18129

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Function(s)

receptor activity calcium ion binding protein binding transcription factor activity, ligand-activated RNA polymerase II transcription factor binding enzyme binding NF-kappaB binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate determination liver development placenta development heart looping morphogenesis of an epithelial sheet inflammatory response to antigenic stimulus pulmonary valve morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter humoral immune response cell cycle arrest Notch signaling pathway multicellular organism development determination of left/right symmetry positive regulation of cell proliferation negative regulation of cell proliferation organ morphogenesis cell growth cell differentiation embryonic limb morphogenesis positive regulation of BMP signaling pathway multicellular organism growth intrahepatic bile duct development wound healing defense response to bacterium myeloid dendritic cell differentiation positive regulation of apoptotic process positive regulation of osteoclast differentiation positive regulation of Ras protein signal transduction bone remodeling regulation of developmental process atrial septum morphogenesis placenta blood vessel development ciliary body morphogenesis left/right axis specification hepatocyte proliferation liver morphogenesis interleukin-4 secretion cholangiocyte proliferation marginal zone B cell differentiation transcription initiation from RNA polymerase II promoter apoptotic process nervous system development stem cell population maintenance hemopoiesis intracellular receptor signaling pathway tissue regeneration negative regulation of apoptotic process Notch signaling involved in heart development

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NOTCH2 Monoclonal Antibody (16F11), PE, eBioscience™

NOTCH2 Antibody (12-5786-82) in Flow

Product Details

12-5786-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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