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Invitrogen
NOTCH2 Monoclonal Antibody (605724)
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FIGURE: 1 / 2
NOTCH2 Antibody (MA5-24274) in ICC/IF
Immunofluorescence analysis of NOTCH2 was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NOTCH2 (605724) Rat Monoclonal Antibody (Product # MA5-24274) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) a d... View More
Product Details
MA5-24274
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
605724
Immunogen
CHO-derived recombinant mouse Notch-2 Leu26-Val528 (predicted)
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610319
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved Notch signaling pathway protein. NOTCH2 is a type 1 transmembrane protein with an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. NOTCH2 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Motch B; Neurogenic locus notch homolog protein 2; Notch 2; Notch gene homolog 2
Gene Aliases: AI853703; N2; Notch2
UniProt ID: (Mouse) O35516
Entrez Gene ID: (Mouse) 18129
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
cell fate determination
liver development
placenta development
heart looping
morphogenesis of an epithelial sheet
inflammatory response to antigenic stimulus
pulmonary valve morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
humoral immune response
cell cycle arrest
Notch signaling pathway
multicellular organism development
determination of left/right symmetry
positive regulation of cell proliferation
negative regulation of cell proliferation
organ morphogenesis
cell growth
cell differentiation
embryonic limb morphogenesis
positive regulation of BMP signaling pathway
multicellular organism growth
intrahepatic bile duct development
wound healing
defense response to bacterium
myeloid dendritic cell differentiation
positive regulation of apoptotic process
positive regulation of osteoclast differentiation
positive regulation of Ras protein signal transduction
bone remodeling
regulation of developmental process
atrial septum morphogenesis
placenta blood vessel development
ciliary body morphogenesis
left/right axis specification
hepatocyte proliferation
liver morphogenesis
interleukin-4 secretion
cholangiocyte proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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