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NOTCH3 Antibody (711007) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), U-87 MG (Lane 2), SH-SY5Y (Lane 3), NTERA-2 (Lane 4), MDA-MB-231 (Lane 5), T-47D (Lane 6), U-2 OS (Lane 7) and A-431 (Lane 8). The blots were probed with Anti-Notch 3 Recombinant Rabbit Superclonal™ Antibody (Product # 711007, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 110 kDa band corresponding to Notch 3 was o... View More
Product Details
711007
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
4HCLC
Immunogen
Peptides corresponding to Human NOTCH3 (aa 2308-2321, 1618-1636, 1803-1816)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633105
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Cat and Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurogenic locus notch homolog protein 3; Notch 3; Notch gene homolog 3; Notch homolog 3
Gene Aliases: AW229011; CADASIL; CADASIL1; CASIL; hpbk; IMF2; LMNS; N3; NOTCH3
UniProt ID: (Human) Q9UM47, (Mouse) Q61982
Entrez Gene ID: (Human) 4854, (Mouse) 18131
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
Notch signaling pathway
Notch receptor processing
forebrain development
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
neuron fate commitment
artery morphogenesis
glomerular capillary formation
cell-cell adhesion
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
negative regulation of cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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