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Invitrogen
NOTCH3 Monoclonal Antibody (HMN3-133), PE, eBioscience™
FIGURE: 1 / 1
NOTCH3 Antibody (12-5763-80) in Flow
Staining of CD4+CD8+ (blue histogram) and CD4-CD8-CD25+CD44- (purple histogram) C57BL/6 thymocytes with 0.5 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (left) or 0.25 µg of Anti-Mouse Notch3 PE (right). Total viable cells were used for analysis.
Product Details
12-5763-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
HMN3-133
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1548667
Product Specific Information
Description: This HMN3-133 monoclonal antibody reacts with mouse Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription.
Notch 3 expression has been demonstrated on regulatory T cells and some thymocyte subsets, including CD4-CD8- and CD8SP cells. In addition, this Notch receptor is prevalent in vascular smooth muscle and the central nervous system. In addition to its role in stem cell hematopoiesis, Notch 3 plays a pivotal role in mammalian T cell lineage commitment and thymocyte development. Specifically, Notch 3 has been implicated in the CD4-CD8- double negative (DN) to CD4+CD8+ double positive transition with greatest expression in DN3 (CD4-CD8-CD25+CD44-) cells. Notch 3 may also be involved in regulating the pre-TCR checkpoint via crosstalk with the NFkappaB and E2A signaling pathways. Finally, overexpression of Notch 3 has been implicated in leukemia.
Applications Reported: This HMN3-133 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HMN3-133 antibody has been tested by flow cytometric analysis of mouse thymoyctes or transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurogenic locus notch homolog protein 3; Notch 3; Notch gene homolog 3
Gene Aliases: AW229011; hpbk; N3; Notch3
UniProt ID: (Mouse) Q61982
Entrez Gene ID: (Mouse) 18131
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
Notch signaling pathway
multicellular organism development
cell differentiation
forebrain development
negative regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
neuron fate commitment
artery morphogenesis
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