Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 8
NOV Antibody (PA5-79750) in ICC/IF
Immunocytochemistry analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (Product # PA5-79750) . NOV/CCN3 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NOV/CCN3 antibody (Product # PA5-79750) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More
Product Details
PA5-79750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3 (334-357aa HTNCPKNNEAFLQELELKTTRGKM).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746865
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell. IHC: human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat kidney tissue. ICC/IF: A431 cell. Flow: U87 cell.
Target Information
NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilm's tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation is correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and cell migration by signaling through specific cell-surface integrins, and by binding to heparin sulfate proteoglycans and to fibulin 1C. NOV has also been reported to exert proangiogenic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CCN family member 3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NovH; Protein NOV homolog
Gene Aliases: CCN3; IBP-9; IGFBP-9; IGFBP9; NOV; NOVH
UniProt ID: (Human) P48745, (Rat) Q9QZQ5
Entrez Gene ID: (Human) 4856, (Rat) 81526
angiogenesis
chondrocyte differentiation
cell-cell signaling
regulation of gene expression
fibroblast migration
negative regulation of myotube differentiation
smooth muscle cell migration
negative regulation of cell growth
cell adhesion mediated by integrin
endothelial cell chemotaxis
negative regulation of NF-kappaB import into nucleus
type B pancreatic cell proliferation
positive regulation of Notch signaling pathway
negative regulation of insulin secretion
smooth muscle cell proliferation
negative regulation of inflammatory response
cell chemotaxis
negative regulation of SMAD protein import into nucleus
negative regulation of cell death
hematopoietic stem cell homeostasis
endothelial cell-cell adhesion
negative regulation of monocyte chemotaxis
negative regulation of chondrocyte proliferation
negative regulation of sensory perception of pain
bone regeneration
regulation of cell growth
cell adhesion
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support