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Invitrogen
NOX5 Polyclonal Antibody
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FIGURE: 1 / 3
NOX5 Antibody (PA5-142647) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of NOX5 in HeLa cells using NOX5 Polyclonal Antibody (Product # PA5-142647). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing staining within the cytoplasm and plasma membrane . The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-EWHPFTISSAPEQKD.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933293
Product Specific Information
Additional Information: This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:8,000.
Target Information
This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NADPH oxidase 5; NOX5zeta
Gene Aliases: NOX5
UniProt ID: (Human) Q96PH1
Entrez Gene ID: (Human) 79400
response to reactive oxygen species
cytokinesis
angiogenesis
endothelial cell proliferation
apoptotic process
cell proliferation
regulation of proton transport
proton transport
superoxide anion generation
regulation of fusion of sperm to egg plasma membrane
cytokine secretion
oxidation-reduction process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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