Proteintech

NPC1 Polyclonal Antibody

NPC1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

NPC1 Antibody (13926-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13926-1-AP (NPC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NPC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

13926-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NPC1 Fusion Protein Ag4946 (23-266 aa encoded by BC063302)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This anti-NPC1 antibody will not cross-react to NPC2, another gene whose defects also result in Niemann-Pick type C disease.

Immunogen sequence: QSCVWYGEC GIAYGDKRYN CEYSGPPKPL PKDGYDLVQE LCPGFFFGNV SLCCDVRQLQ TLKDNLQLPL QFLSRCPSCF YNLLNLFCEL TCSPRQSQFL NVTATEDYVD PVTNQTKTNV KELQYYVGQG FANAMYNACR DVEAPSSNDK ALGLLCGKDA DACNATNWIE YMFNKDNGQA PFTITPVFSD FPVHGMEPMN NATKGCDESV DEVTAPCSCQ DCSIVCGPKP QPPPPPAPWT ILGLD (23-266 aa encoded by B C063302)

Target Information

Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.

Science China. Life sciences

Li G,Su B,Fu P,Bai Y,Ding G,Li D,Wang J,Yang G,Chu B

Tue Feb 01 00:00:00 UTC 2022

A Cytological Atlas of the Human Liver Proteome from PROTEOME^SKY-LIVER^Hu 2.0, a Publicly Available Database.

Journal of proteome research

Lao Y,Li Y,Wang W,Ren L,Qian X,He F,Chen X,Jiang Y

Fri Aug 05 00:00:00 UTC 2022

Enhancing the Endocytosis of Phosphatidylserine-Containing Liposomes through Tim4 by Modulation of Membrane Fluidity.

Molecular pharmaceutics

Hirose M,Ueno T,Nagumo H,Sato Y,Sakai-Kato K

Mon Jan 03 00:00:00 UTC 2022

Proteomic profile of human dental follicle stem cells and apical papilla stem cells.

Journal of proteomics

Lei T,Zhang X,Chen P,Li Q,Du H

Sat Jan 16 00:00:00 UTC 2021

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.

Nature communications

Zhu Y,Feng F,Hu G,Wang Y,Yu Y,Zhu Y,Xu W,Cai X,Sun Z,Han W,Ye R,Qu D,Ding Q,Huang X,Chen H,Xu W,Xie Y,Cai Q,Yuan Z,Zhang R

Thu Feb 11 00:00:00 UTC 2021

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

The EMBO journal

Miyamoto T,Hosoba K,Itabashi T,Iwane AH,Akutsu SN,Ochiai H,Saito Y,Yamamoto T,Matsuura S

Wed Jun 17 00:00:00 UTC 2020

Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.

Proceedings of the National Academy of Sciences of the United States of America

Petit CS,Lee JJ,Boland S,Swarup S,Christiano R,Lai ZW,Mejhert N,Elliott SD,McFall D,Haque S,Huang EJ,Bronson RT,Harper JW,Farese RV,Walther TC

Tue May 12 00:00:00 UTC 2020

Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P₂ and extended synaptotagmins.

Science China. Life sciences

Xiao J,Luo J,Hu A,Xiao T,Li M,Kong Z,Jiang L,Zhou Z,Liao Y,Xie C,Chu B,Miao H,Li B,Shi X,Song BL

Sun Sep 01 00:00:00 UTC 2019

The delayed effects of antibiotics in type 2 diabetes, friend or foe?

The Journal of endocrinology

Fu L,Qiu Y,Shen L,Cui C,Wang S,Wang S,Xie Y,Zhao X,Gao X,Ning G,Nie A,Gu Y

Wed Aug 01 00:00:00 UTC 2018

The delayed effects of antibiotics in type 2 diabetes, friend or foe?

The Journal of endocrinology

Fu L,Qiu Y,Shen L,Cui C,Wang S,Wang S,Xie Y,Zhao X,Gao X,Ning G,Nie A,Gu Y

Wed Aug 01 00:00:00 UTC 2018

Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking.

International journal of biological sciences

Lyu J,Yang EJ,Head SA,Ai N,Zhang B,Wu C,Li RJ,Liu Y,Chakravarty H,Zhang S,Tam KY,Dang Y,Kwon HJ,Ge W,Liu JO,Shim JS

Tue Aug 20 00:00:00 UTC 2019

Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking.

International journal of biological sciences

Lyu J,Yang EJ,Head SA,Ai N,Zhang B,Wu C,Li RJ,Liu Y,Chakravarty H,Zhang S,Tam KY,Dang Y,Kwon HJ,Ge W,Liu JO,Shim JS

Tue Aug 20 00:00:00 UTC 2019

The non-canonical NF-κB pathway promotes NPC2 expression and regulates intracellular cholesterol trafficking.

Science China. Life sciences

Liao Y,Wei J,Wang J,Shi X,Luo J,Song BL

Mon Oct 01 00:00:00 UTC 2018

Pharmacological blockade of cholesterol trafficking by cepharanthine in endothelial cells suppresses angiogenesis and tumor growth.

Cancer letters

Lyu J,Yang EJ,Head SA,Ai N,Zhang B,Wu C,Li RJ,Liu Y,Yang C,Dang Y,Kwon HJ,Ge W,Liu JO,Shim JS

Tue Nov 28 00:00:00 UTC 2017

Pharmacological blockade of cholesterol trafficking by cepharanthine in endothelial cells suppresses angiogenesis and tumor growth.

Cancer letters

Lyu J,Yang EJ,Head SA,Ai N,Zhang B,Wu C,Li RJ,Liu Y,Yang C,Dang Y,Kwon HJ,Ge W,Liu JO,Shim JS

Tue Nov 28 00:00:00 UTC 2017

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.

Cell reports

Wei J,Zhang YY,Luo J,Wang JQ,Zhou YX,Miao HH,Shi XJ,Qu YX,Xu J,Li BL,Song BL

Tue Jun 27 00:00:00 UTC 2017

The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.

Cell reports

Wei J,Zhang YY,Luo J,Wang JQ,Zhou YX,Miao HH,Shi XJ,Qu YX,Xu J,Li BL,Song BL

Tue Jun 27 00:00:00 UTC 2017

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 UTC 2016

VNN1 promotes atherosclerosis progression in apoE-/- mice fed a high-fat/high-cholesterol diet.

Journal of lipid research

Hu YW,Wu SG,Zhao JJ,Ma X,Lu JB,Xiu JC,Zhang Y,Huang C,Qiu YR,Sha YH,Gao JJ,Wang YC,Li SF,Zhao JY,Zheng L,Wang Q

Mon Aug 01 00:00:00 UTC 2016

Cholesterol transport through lysosome-peroxisome membrane contacts.

Cell

Chu BB,Liao YC,Qi W,Xie C,Du X,Wang J,Yang H,Miao HH,Li BL,Song BL

Thu Apr 09 00:00:00 UTC 2015

Cholesterol transport through lysosome-peroxisome membrane contacts.

Cell

Chu BB,Liao YC,Qi W,Xie C,Du X,Wang J,Yang H,Miao HH,Li BL,Song BL

Thu Apr 09 00:00:00 UTC 2015

Cholesterol trafficking is required for mTOR activation in endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America

Xu J,Dang Y,Ren YR,Liu JO

Tue Mar 09 00:00:00 UTC 2010

Cholesterol trafficking is required for mTOR activation in endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America

Xu J,Dang Y,Ren YR,Liu JO

Tue Mar 09 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: FLJ98532; Niemann Pick type C1; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; NPC intracellular cholesterol transporter 1; sphingomyelinosis; truncated Niemann-Pick C1

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Gene Aliases: A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; NPC; NPC1; spm

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UniProt ID: (Human) O15118, (Mouse) O35604

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Entrez Gene ID: (Human) 4864, (Mouse) 18145

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity protein binding sterol transporter activity cholesterol binding hedgehog receptor activity

Process(es)

protein glycosylation lipid metabolic process endocytosis autophagy adult walking behavior steroid metabolic process cholesterol metabolic process negative regulation of macroautophagy cholesterol transport membrane raft organization cholesterol efflux response to drug cholesterol homeostasis response to cadmium ion viral entry into host cell negative regulation of cell death cellular response to steroid hormone stimulus cellular response to low-density lipoprotein particle stimulus establishment of protein localization to membrane positive regulation of cholesterol homeostasis lysosomal transport signal transduction bile acid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPC1 Polyclonal Antibody

NPC1 Antibody (13926-1-AP) in ICC/IF

Product Details

13926-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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