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Invitrogen
NPC1 Recombinant Rabbit Monoclonal Antibody (JB87-33)
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FIGURE: 1 / 8
NPC1 Antibody (MA5-34694) in ICC/IF
Immunofluorescent analysis of Niemann Pick C1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), followed by DAPI (blue).
Product Details
MA5-34694
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB87-33
Immunogen
Recombinant protein within Human Niemann Pick C1 aa 1,160-1,278
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848602
Product Specific Information
Positive Control: SiHa, A549, HepG2, PC-3M, Rat kidney tissue, human kidney tissue, mouse testis tissue, SH-SY-5Y.
Target Information
Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ98532; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; NPC intracellular cholesterol transporter 1; truncated Niemann-Pick C1
Gene Aliases: NPC; NPC1
UniProt ID: (Human) O15118
Entrez Gene ID: (Human) 4864
protein glycosylation
endocytosis
autophagy
lysosomal transport
signal transduction
adult walking behavior
cholesterol metabolic process
bile acid metabolic process
negative regulation of macroautophagy
cholesterol transport
membrane raft organization
cholesterol efflux
response to drug
cholesterol homeostasis
response to cadmium ion
viral entry into host cell
negative regulation of cell death
cellular response to steroid hormone stimulus
cellular response to low-density lipoprotein particle stimulus
establishment of protein localization to membrane
positive regulation of cholesterol homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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