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NPC2 Antibody (PA5-143858) in ICC/IF
Immunocytochemistry analysis of NPC2 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in NPC2 Polyclonal antibody (Product # PA5-143858) using a dilution of 2 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for... View More
Product Details
PA5-143858
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Niemann Pick C2/NPC2 recombinant protein (Position: E20-L151).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075072
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human U20S whole cell. IHC: human tonsil tissue, mouse spleen tissue. ICC/IF: A431 cell. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymal protein 1; Epididymal secretory protein E1; He1; Human epididymis-specific protein 1; mE1; MGC1333; Niemann Pick type C2 protein homolog; Niemann-Pick disease type C2 protein; Niemann-Pick disease, type C2; NP-C2; NPC intracellular cholesterol transporter 2; tissue-specific secretory protein
Gene Aliases: 2700012J19Rik; AA408070; AU045843; EDDM1; HE1; NPC2
UniProt ID: (Human) P61916, (Mouse) Q9Z0J0
Entrez Gene ID: (Human) 10577, (Mouse) 67963
cholesterol metabolic process
response to virus
phospholipid transport
regulation of isoprenoid metabolic process
cholesterol transport
intracellular sterol transport
intracellular cholesterol transport
cholesterol efflux
cholesterol homeostasis
glycolipid transport
lipid metabolic process
steroid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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