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Invitrogen
NPFF2 Polyclonal Antibody
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FIGURE: 1 / 1
NPFF2 Antibody (PA5-88020) in WB
Western blot analysis of NPFF2 in extracts of various cell lines. Samples were incubated with NPFF2 Polyclonal antibody (Product # PA5-88020) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88020
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human NPFFR2 (NP_0048762)
Conjugate
Form
Liquid
Concentration
1.69 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804591
Product Specific Information
Immunogen sequence: MNSFFGTPAA SWCLLESDVS SAPDKEAGRE RRALSVQQRG GPAWSGSLEW SRQSAGDRRR LGLSRQTAKS SWSRSRDRTC CCRRAWWILV PAADRARRER FIMNEKWDTN SSENWHPIWN VNDTKHHLYS DINITYVNYY; Positive Samples: Mouse brain, Rat brain; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein-coupled receptor 74; G-protein coupled receptor 74; Neuropeptide FF receptor 2; Neuropeptide G-protein coupled receptor; Neuropeptide NPFF receptor; NPFF-R2; NPFF2 receptor; NPGP receptor
Gene Aliases: Gpr74; HG31; NPFF2; Npffr2; Npgpr
UniProt ID: (Rat) Q9EQD2, (Mouse) Q924H0
Entrez Gene ID: (Rat) 78964, (Mouse) 104443
neuropeptide signaling pathway
chemical synaptic transmission
cellular response to hormone stimulus
regulation of MAPK cascade
regulation of adenylate cyclase activity
response to peptide
regulation of cAMP-dependent protein kinase activity
signal transduction
G-protein coupled receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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