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NPHP4 Polyclonal Antibody

NPHP4 Antibody in Immunocytochemistry (ICC/IF)

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NPHP4 Antibody (13812-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

NPHP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

13812-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 8 publications 8 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NPHP4 Fusion Protein Ag4875 (1-350 aa encoded by BC040520)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNDWHRIFT QNVLVPPHPQ RARQPWKEST AFQCVLKWLD GPVIRQGVLE VLSEVECHLR VSFFDVTYRH FFGRTWKTTV KPTKRPPSRI VFNEPLYFHT SLNHPHIVAV VEVVAEGKKR DGSLQTLSCG FGILRIFSNQ PDSPISASQD KRLRLYHGTP RALLHPLLQD PAEQNRHMTL IENCSLQYTL KPHPALEPAF HLLPENLLVS GLQQIPGLLP AHGESGDALR KPRLQKPITG HLDDLFFTLY PSLEKFEEEL LELHVQDHFQ EGCGPLDGGA LEILERRLRV GVHNGLGFVQ RPQVVVLVPE MDVALTRSAS FSRKVVSSSK TSSGSQALVL RSRLRLPEMV G (1-350 aa encoded by BC040520)

Target Information

This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E.

Molecular biology of the cell

Dewees SI,Vargová R,Hardin KR,Turn RE,Devi S,Linnert J,Wolfrum U,Caspary T,Eliáš M,Kahn RA

Fri Apr 01 00:00:00 UTC 2022

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Science advances

Chen C,Xu Q,Zhang Y,Davies BA,Huang Y,Katzmann DJ,Harris PC,Hu J,Ling K

Tue Jun 01 00:00:00 UTC 2021

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 UTC 2018

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Biophysical journal

Weng RR,Yang TT,Huang CE,Chang CW,Wang WJ,Liao JC

Tue Jul 17 00:00:00 UTC 2018

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Biophysical journal

Weng RR,Yang TT,Huang CE,Chang CW,Wang WJ,Liao JC

Tue Jul 17 00:00:00 UTC 2018

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Nature communications

Tu HQ,Qin XH,Liu ZB,Song ZQ,Hu HB,Zhang YC,Chang Y,Wu M,Huang Y,Bai YF,Wang G,Han QY,Li AL,Zhou T,Liu F,Zhang XM,Li HY

Tue Dec 11 00:00:00 UTC 2018

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Nature communications

Tu HQ,Qin XH,Liu ZB,Song ZQ,Hu HB,Zhang YC,Chang Y,Wu M,Huang Y,Bai YF,Wang G,Han QY,Li AL,Zhou T,Liu F,Zhang XM,Li HY

Tue Dec 11 00:00:00 UTC 2018

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Genetics

Kazatskaya A,Kuhns S,Lambacher NJ,Kennedy JE,Brear AG,McManus GJ,Sengupta P,Blacque OE

Fri Dec 01 00:00:00 UTC 2017

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Genetics

Kazatskaya A,Kuhns S,Lambacher NJ,Kennedy JE,Brear AG,McManus GJ,Sengupta P,Blacque OE

Fri Dec 01 00:00:00 UTC 2017

Lineage specificity of primary cilia in the mouse embryo.

Nature cell biology

Bangs FK,Schrode N,Hadjantonakis AK,Anderson KV

Sun Feb 01 00:00:00 UTC 2015

Lineage specificity of primary cilia in the mouse embryo.

Nature cell biology

Bangs FK,Schrode N,Hadjantonakis AK,Anderson KV

Sun Feb 01 00:00:00 UTC 2015

Visualizing renal primary cilia.

Nephrology (Carlton, Vic.)

Deane JA,Verghese E,Martelotto LG,Cain JE,Galtseva A,Rosenblum ND,Watkins DN,Ricardo SD

Fri Mar 01 00:00:00 UTC 2013

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 UTC 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 UTC 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Nephrocystin-4; Nephroretinin; POC10 centriolar protein homolog

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Gene Aliases: 4930564O18Rik; KIAA0673; nmf192; NPHP4; POC10; SLSN4

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UniProt ID: (Human) O75161, (Mouse) P59240

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Entrez Gene ID: (Dog) 489625, (Human) 261734, (Mouse) 260305

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Function(s)

structural molecule activity protein binding

Process(es)

signal transduction visual behavior single organismal cell-cell adhesion actin cytoskeleton organization sperm motility hippo signaling photoreceptor cell outer segment organization photoreceptor cell maintenance retina development in camera-type eye negative regulation of canonical Wnt signaling pathway positive regulation of bicellular tight junction assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPHP4 Polyclonal Antibody

NPHP4 Antibody (13812-1-AP) in ICC/IF

Product Details

13812-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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