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Invitrogen
NPY Monoclonal Antibody (8)
FIGURE: 1 / 8
NPY Antibody (ABS 028-08-02) in ICC/IF
Immunofluorescence analysis of NPY was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NPY Monoclonal Antibody (8) (Product # ABS 028-08-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), ... View More
Product Details
ABS 028-08-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Pig
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8
Immunogen
Synthetic peptide made to human/rat NPY.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1077304
Product Specific Information
ABS 028-08-02 detects Neuropeptide Y in human and rat samples.
ABS 028-08-02 has been successfully used in ELISA and immunohistochemistry (paraffin) procedures.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase, regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Neuropeptide-Y; OTTHUMP00000201947; prepro-neuropeptide Y; Pro-neuropeptide Y; pro-neuropeptide Y-like
Gene Aliases: 0710005A05Rik; NPY; NPY02; PYY4; RATNPY; RATNPY02
UniProt ID: (Human) P01303, (Mouse) P57774, (Rat) P07808
Entrez Gene ID: (Rat) 100912228, (Human) 4852, (Mouse) 109648, (Rat) 24604
neuropeptide signaling pathway
cell-cell signaling
feeding behavior
regulation of blood pressure
regulation of appetite
chemical synaptic transmission
adult feeding behavior
central nervous system neuron development
cerebral cortex development
neuron projection development
positive regulation of appetite
negative regulation of blood pressure
sensory perception of taste
calcium ion transport
movement of cell or subcellular component
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
digestion
behavior
blood circulation
cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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