Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
NR0B1 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 4
NR0B1 Antibody (PA5-18716) in ICC/IF
Immunocytochemistry analysis of NR0B1 using NR0B1 Polyclonal Antibody (Product # PA5-18716) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-18716
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (CGEDHPQQGSTLY) corresponding to the internal amino acids of NR0B1 (aa 202-215).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10988362
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DAX 1; DSS-AHC critical region on the X chromosome protein 1; nuclear hormone receptor; Nuclear receptor DAX-1; Nuclear receptor subfamily 0 group B member 1; nuclear receptor subfamily 0, group B, member 1; R0B1
Gene Aliases: AHC; AHCH; AHX; DAX-1; DAX1; DSS; GTD; HHG; NR0B1; NROB1; SRXY2
UniProt ID: (Human) P51843
Entrez Gene ID: (Human) 190
DNA binding
steroid hormone receptor activity
transcription corepressor activity
RNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
transcription factor binding
protein domain specific binding
DNA hairpin binding
steroid hormone receptor binding
protein homodimerization activity
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
steroid biosynthetic process
spermatogenesis
protein localization
gonad development
male gonad development
hypothalamus development
pituitary gland development
male sex determination
adrenal gland development
intracellular receptor signaling pathway
negative regulation of intracellular steroid hormone receptor signaling pathway
Leydig cell differentiation
response to immobilization stress
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
Sertoli cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support