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NR0B2 Antibody (PA5-76632) in WB
Western blot analysis of NR0B2 in extracts of Mouse pancreas. Samples were incubated with NR0B2 polyclonal antibody (Product # PA5-76632) using a dilution of 1:500 followed by HRP Goat Anti-Rabbit IgG at 1:10,000 dilution. Lysates/proteins: 25 µg per Lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-76632
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human NR0B2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2720359
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nr0b2a; nuclear receptor SHP; Nuclear receptor subfamily 0 group B member 2; nuclear receptor subfamily 0, group B, member 2; Orphan nuclear receptor SHP; Small heterodimer partner; small heterodimer partner homologue
Gene Aliases: NR0B2; SHP; SHP-1; SHP1
UniProt ID: (Human) Q15466, (Rat) P97947, (Mouse) Q62227
Entrez Gene ID: (Human) 8431, (Rat) 117274, (Mouse) 23957
DNA binding
steroid hormone receptor activity
transcription corepressor activity
protein binding
transcription factor binding
protein domain specific binding
protein complex binding
protein homodimerization activity
peroxisome proliferator activated receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
transcription factor activity, sequence-specific DNA binding
retinoic acid receptor binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
response to glucose
positive regulation of gene expression
negative regulation of gene expression
organ regeneration
positive regulation of insulin secretion
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
Notch signaling pathway
cholesterol metabolic process
regulation of transcription, DNA-templated
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