Proteintech

NR1D1 Polyclonal Antibody

NR1D1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

NR1D1 Antibody (14506-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NR1D1 Antibody in Immunoprecipitation (IP)

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Product Details

14506-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

ChIP assay (ChIP)

Assay-dependent

View 3 publications 3 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NR1D1 Fusion Protein Ag5964 (1-350 aa encoded by BC056148)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

Immunogen sequence: MTTLDSNNN TGGVITYIGS SGSSPSRTSP ESLYSDNSNG SFQSLTQGCP TYFPPSPTGS LTQDPARSFG SIPPSLSDDG SPSSSSSSSS SSSSFYNGSP PGSLQVAMED SSRVSPSKST SNITKLNGMV LLCKVCGDVA SGFHYGVHAC EGCKGFFRRS IQQNIQYKRC LKNENCSIVR INRNRCQQCR FKKCLSVGMS RDAVRFGRIP KREKQRMLAE MQSAMNLANN QLSSQCPLET SPTQHPTPGP MGPSPPPAPV PSPLVGFSQF PQQLTPPRSP SPEPTVEDVI SQVARAHREI FTYAHDKLGS SPGNFNANHA SGSPPATTPH RWENQGCPPA PNDNNTLAAQ R (1-350 aa encoded by BC056148)

Target Information

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA Nron deficiency protects mice from diet-induced adiposity and hepatic steatosis.

Metabolism: clinical and experimental

Liu B,Zhong Y,Huang D,Yang L,Wang P,Yang L,Zhang F,Li X,Liang M,Huang K,Du M

Wed Nov 01 00:00:00 UTC 2023

m⁶A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis.

Pharmacological research

Chen L,Xia S,Wang F,Zhou Y,Wang S,Yang T,Li Y,Xu M,Zhou Y,Kong D,Zhang Z,Shao J,Xu X,Zhang F,Zheng S

Wed Mar 01 00:00:00 UTC 2023

m⁶A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis.

Pharmacological research

Chen L,Xia S,Wang F,Zhou Y,Wang S,Yang T,Li Y,Xu M,Zhou Y,Kong D,Zhang Z,Shao J,Xu X,Zhang F,Zheng S

Wed Mar 01 00:00:00 UTC 2023

m⁶A methylation-induced NR1D1 ablation disrupts the HSC circadian clock and promotes hepatic fibrosis.

Pharmacological research

Chen L,Xia S,Wang F,Zhou Y,Wang S,Yang T,Li Y,Xu M,Zhou Y,Kong D,Zhang Z,Shao J,Xu X,Zhang F,Zheng S

Wed Mar 01 00:00:00 UTC 2023

Fluoxetine Decreases Phagocytic Function via REV-ERBα in Microglia.

Neurochemical research

Jang DY,Yang B,You MJ,Rim C,Kim HJ,Sung S,Kwon MS

Sun Jan 01 00:00:00 UTC 2023

Regulation of BCRP expression and sulfasalazine pharmacokinetics by the nuclear receptor REV-ERBα.

Xenobiotica; the fate of foreign compounds in biological systems

Wu C,Xiao Y,Wu C,Xie D,Luo M,Yao D,Chen M,Lu D

Wed Mar 01 00:00:00 UTC 2023

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

Theranostics

Wang S,Lin Y,Gao L,Yang Z,Lin J,Ren S,Li F,Chen J,Wang Z,Dong Z,Sun P,Wu B

Thu Apr 21 00:00:00 UTC 2022

The nuclear receptor REV-ERBα regulates CYP2E1 expression and acetaminophen hepatotoxicity.

Xenobiotica; the fate of foreign compounds in biological systems

Zhang L,Zhang F,Xiao Y,Du J,Zhang X,Chen M,Wu B

Wed Jun 01 00:00:00 UTC 2022

BMAL1 regulates Propionibacterium acnes-induced skin inflammation via REV-ERBα in mice.

International journal of biological sciences

Li F,Lin L,He Y,Sun G,Dong D,Wu B

Thu Apr 14 00:00:00 UTC 2022

The actin cytoskeleton-MRTF/SRF cascade transduces cellular physical niche cues to entrain the circadian clock.

Journal of cell science

Xiong X,Li W,Nam J,Qu M,Kay SA,Ma K

Sat Oct 01 00:00:00 UTC 2022

The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.

Journal of neuroinflammation

Kou L,Chi X,Sun Y,Han C,Wan F,Hu J,Yin S,Wu J,Li Y,Zhou Q,Zou W,Xiong N,Huang J,Xia Y,Wang T

Mon Jun 06 00:00:00 UTC 2022

Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.

Arthritis research & therapy

Song X,Zhao M,Tang J,Ma T,Bai H,Wang X,Liu L,Li T,Xu X,Sheng X,Zhao B,Wang Y,Wang T,Guo Y,Zhang X,Gao L

Tue Jun 28 00:00:00 UTC 2022

Rev-erbα exacerbates hepatic steatosis in alcoholic liver diseases through regulating autophagy.

Cell & bioscience

Liu Q,Xu L,Wu M,Zhou Y,Yang J,Huang C,Xu T,Li J,Zhang L

Sat Jul 10 00:00:00 UTC 2021

Dietary Conjugated Linoleic Acid Modulates the Hepatic Circadian Clock Program via PPARα/REV-ERBα-Mediated Chromatin Modification in Mice.

Frontiers in nutrition

Liu HY,Gu H,Li Y,Hu P,Yang Y,Li K,Li H,Zhang K,Zhou B,Wu H,Bao W,Cai D

Tue Nov 02 00:00:00 UTC 2021

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Nature communications

Liu Y,Zhang Y,Li T,Han J,Wang Y

Thu Jan 30 00:00:00 UTC 2020

The Nuclear Receptor and Clock Repressor Rev-erbα Suppresses Myogenesis.

Scientific reports

Chatterjee S,Yin H,Li W,Lee J,Yechoor VK,Ma K

Thu Mar 14 00:00:00 UTC 2019

NR1D1 Recruitment to Sites of DNA Damage Inhibits Repair and Is Associated with Chemosensitivity of Breast Cancer.

Cancer research

Ka NL,Na TY,Na H,Lee MH,Park HS,Hwang S,Kim IY,Seong JK,Lee MO

Mon May 01 00:00:00 UTC 2017

NR1D1 Recruitment to Sites of DNA Damage Inhibits Repair and Is Associated with Chemosensitivity of Breast Cancer.

Cancer research

Ka NL,Na TY,Na H,Lee MH,Park HS,Hwang S,Kim IY,Seong JK,Lee MO

Mon May 01 00:00:00 UTC 2017

NR1D1 Recruitment to Sites of DNA Damage Inhibits Repair and Is Associated with Chemosensitivity of Breast Cancer.

Cancer research

Ka NL,Na TY,Na H,Lee MH,Park HS,Hwang S,Kim IY,Seong JK,Lee MO

Mon May 01 00:00:00 UTC 2017

NR1D1 Recruitment to Sites of DNA Damage Inhibits Repair and Is Associated with Chemosensitivity of Breast Cancer.

Cancer research

Ka NL,Na TY,Na H,Lee MH,Park HS,Hwang S,Kim IY,Seong JK,Lee MO

Mon May 01 00:00:00 UTC 2017

NR1D1 enhances oxidative DNA damage by inhibiting PARP1 activity.

Molecular and cellular endocrinology

Ka NL,Na TY,Lee MO

Sun Oct 15 00:00:00 UTC 2017

NR1D1 enhances oxidative DNA damage by inhibiting PARP1 activity.

Molecular and cellular endocrinology

Ka NL,Na TY,Lee MO

Sun Oct 15 00:00:00 UTC 2017

NR1D1 enhances oxidative DNA damage by inhibiting PARP1 activity.

Molecular and cellular endocrinology

Ka NL,Na TY,Lee MO

Sun Oct 15 00:00:00 UTC 2017

NR1D1 enhances oxidative DNA damage by inhibiting PARP1 activity.

Molecular and cellular endocrinology

Ka NL,Na TY,Lee MO

Sun Oct 15 00:00:00 UTC 2017

Deletion of exons 3 and 4 in the mouse Nr1d1 gene worsens high-fat diet-induced hepatic steatosis.

Life sciences

Na H,Lee H,Lee MH,Lim HJ,Kim HJ,Jeon Y,Kang HL,Lee MO

Thu Dec 01 00:00:00 UTC 2016

Deletion of exons 3 and 4 in the mouse Nr1d1 gene worsens high-fat diet-induced hepatic steatosis.

Life sciences

Na H,Lee H,Lee MH,Lim HJ,Kim HJ,Jeon Y,Kang HL,Lee MO

Thu Dec 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: BD73; EAR-1; EAR-1R; nuclear receptor protein; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; orphan nuclear receptor; rev-erb alpha; Rev-erba; rev-erbA alpha; rev-erbA(alpha); Rev-erbA-alpha; Rev-ErbA-alpha protein; Rev-ErbAalpha; REV-ERBalpha; Reverbaalpha; RVR; V-erbA-related protein 1

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Gene Aliases: A530070C09Rik; ear-1; EAR1; HREV; NR1D1; R75201; THRA1; THRAL

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UniProt ID: (Human) P20393, (Mouse) Q3UV55

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Entrez Gene ID: (Human) 9572, (Mouse) 217166

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription corepressor binding steroid hormone receptor activity transcription corepressor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding heme binding transcription regulatory region DNA binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter glycogen biosynthetic process transcription initiation from RNA polymerase II promoter circadian rhythm proteasomal protein catabolic process negative regulation of receptor biosynthetic process regulation of lipid metabolic process cell differentiation intracellular receptor signaling pathway circadian regulation of gene expression negative regulation of toll-like receptor 4 signaling pathway regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter regulation of circadian rhythm steroid hormone mediated signaling pathway response to leptin regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated circadian temperature homeostasis regulation of insulin secretion involved in cellular response to glucose stimulus regulation of type B pancreatic cell proliferation positive regulation of bile acid biosynthetic process cellular response to lipopolysaccharide regulation of cholesterol homeostasis positive regulation of cholesterol homeostasis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NR1D1 Polyclonal Antibody

NR1D1 Antibody (14506-1-AP) in ICC/IF

Product Details

14506-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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