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Invitrogen
NR1D1 Recombinant Rabbit Monoclonal Antibody (9I2H1)
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FIGURE: 1 / 3
NR1D1 Antibody (MA5-42757) in WB
Western blot analysis of NR1D1 in extracts of various cell lines. Samples were incubated with NR1D1 Monoclonal antibody (Product # MA5-42757) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-42757
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9I2H1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 280-360 of human Rev-Erbα/NR1D1 (NP_0683701)
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911898
Product Specific Information
Positive test controls include: HepG2, Mouse liver, Rat brain, Rat lung.
Immunogen sequence: SPEPTVEDVI SQVARAHREI FTYAHDKLGS SPGNFNANHA SGSPPATTPH RWENQGCPPA PNDNNTLAAQ RHNEALNGLR Q
Target Information
This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BD73; EAR-1; EAR-1R; nuclear receptor protein; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; orphan nuclear receptor; rev-erb alpha; Rev-erba; rev-erbA alpha; rev-erbA(alpha); Rev-erbA-alpha; Rev-ErbA-alpha protein; Rev-ErbAalpha; REV-ERBalpha; Reverbaalpha; RVR; V-erbA-related protein 1; V-erbA-related protein EAR-1
Gene Aliases: A530070C09Rik; ear-1; EAR1; HREV; NR1D1; R75201; REV-ERBAALPHA; THRA1; THRAL
UniProt ID: (Human) P20393, (Rat) Q63503, (Mouse) Q3UV55
Entrez Gene ID: (Human) 9572, (Rat) 252917, (Mouse) 217166
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription corepressor binding
steroid hormone receptor activity
transcription corepressor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
heme binding
transcription regulatory region DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
glycogen biosynthetic process
transcription initiation from RNA polymerase II promoter
circadian rhythm
proteasomal protein catabolic process
negative regulation of receptor biosynthetic process
regulation of lipid metabolic process
cell differentiation
intracellular receptor signaling pathway
circadian regulation of gene expression
negative regulation of toll-like receptor 4 signaling pathway
regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter
regulation of circadian rhythm
steroid hormone mediated signaling pathway
response to leptin
regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
circadian temperature homeostasis
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of type B pancreatic cell proliferation
positive regulation of bile acid biosynthetic process
cellular response to lipopolysaccharide
regulation of cholesterol homeostasis
positive regulation of cholesterol homeostasis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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