OriGene

NR1H3 Monoclonal Antibody (OTI1A5), TrueMAB™

View all (21) LXR alpha antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

CF805015

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1A5

Immunogen

Human recombinant protein fragment corresponding to amino acids 69-329 of human NR1H3 produced in E.coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Its interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides. NR1H3 plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8. It also interplays functionally with RORA for the regulation of genes involves in liver metabolism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: liver X nuclear receptor alpha variant 1; Liver X receptor alpha; LXRb; NER; NERI; NR1H2; Nuclear receptor subfamily 1 group H member 3; nuclear receptor subfamily 1, group H, member 3; Oxysterols receptor LXR-alpha

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Gene Aliases: LXR-a; LXRA; NR1H3; RLD-1

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UniProt ID: (Human) Q13133

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Entrez Gene ID: (Human) 10062

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Function(s)
DNA binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding cholesterol binding sterol response element binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter negative regulation of macrophage derived foam cell differentiation positive regulation of triglyceride biosynthetic process positive regulation of receptor biosynthetic process positive regulation of cholesterol efflux negative regulation of cholesterol storage intracellular receptor signaling pathway positive regulation of cellular protein metabolic process negative regulation of lipid transport positive regulation of cholesterol transport response to progesterone positive regulation of toll-like receptor 4 signaling pathway cholesterol homeostasis regulation of circadian rhythm negative regulation of macrophage activation apoptotic cell clearance steroid hormone mediated signaling pathway positive regulation of fatty acid biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of pinocytosis negative regulation of inflammatory response positive regulation of lipoprotein lipase activity lipid homeostasis sterol homeostasis negative regulation of interferon-gamma-mediated signaling pathway triglyceride homeostasis cellular response to lipopolysaccharide negative regulation of pancreatic juice secretion negative regulation of secretion of lysosomal enzymes regulation of cholesterol homeostasis positive regulation of cholesterol homeostasis
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