Invitrogen

NR5A1 Monoclonal Antibody (N1665)

9 Published Figures

19 References

View all (37) NR5A1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (9)

FIGURE: 1 / 10

NR5A1 Antibody (434200) in WB

Western blot was performed using NR5A1 Monoclonal Antibody (Product # 434200) and a 50 kDa band corresponding to NR5A1 was observed in the cell line and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), Hek-293 (Lane 2), tissue extracts of Mouse Ovary (Lane 3), Mouse Heart (Lane 4) and Mouse Prostate (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blo... View More

NR5A1 Antibody in Immunocytochemistry (ICC/IF)

NR5A1 Antibody in Immunohistochemistry (IHC)

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Product Details

434200

Applications

Tested Dilution

Publications

Western Blot (WB)

3 µg/mL

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

0.1 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N1665

Immunogen

BALB/c mouse immunized with E.coli-expressed recombinant human SF-1 (218-461aa).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

physiological saline

Contains

0.1% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532209

Product Specific Information

This antibody specifically recognizes human SF-1 and cross reacts with mouse and rat SF-1.

Target Information

Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishment of a pure culture of immature Sertoli cells by PDGFRA staining and cell sorting.

Molecular reproduction and development

Malolina EA,Galiakberova AA,Dashinimaev EB,Kulibin AY

434200 was used in Immunocytochemistry to establish a pure Sertoli cell culture from immature mouse testes.

Sun May 01 00:00:00 UTC 2022

Gonadotroph tumours with a low SF-1 labelling index are more likely to recur and are associated with enrichment of the PI3K-AKT pathway.

Neuropathology and applied neurobiology

Hickman RA,Bruce JN,Otten M,Khandji AG,Flowers XE,Siegelin M,Lopes B,Faust PL,Freda PU

Published figure using NR5A1 monoclonal antibody (Product # 434200) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

Pre- and postoperative ⁶⁸ Ga-DOTATOC positron emission tomography for hormone-secreting pituitary neuroendocrine tumours.

Clinical endocrinology

Tjörnstrand A,Casar-Borota O,Heurling K,Schöll M,Gjertsson P,Ragnarsson O,Filipsson Nyström H

434200 was used in Immunohistochemistry to show that corticotroph tumours had a significantly lower uptake compared to the pituitary gland but without a distinct increased tumour uptake the clinical postoperative value is limited.

Tue Jun 01 00:00:00 UTC 2021

Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells.

eLife

Russell JP,Lim X,Santambrogio A,Yianni V,Kemkem Y,Wang B,Fish M,Haston S,Grabek A,Hallang S,Lodge EJ,Patist AL,Schedl A,Mollard P,Nusse R,Andoniadou CL

Published figure using NR5A1 monoclonal antibody (Product # 434200) in Immunocytochemistry

Tue Jan 05 00:00:00 UTC 2021

Essential and sex-specific effects of mGluR5 in ventromedial hypothalamus regulating estrogen signaling and glucose balance.

Proceedings of the National Academy of Sciences of the United States of America

Fagan MP,Ameroso D,Meng A,Rock A,Maguire J,Rios M

434200 was used in Immunohistochemistry (PFA fixed) to show that mGluR5 depletion in SF1 neurons in female but not in male mice leads to reduced activity of these cells as well as deficient glucose and lipid balance control.

Tue Aug 11 00:00:00 UTC 2020

Digital analysis of hormonal immunostaining in pituitary adenomas classified according to WHO 2017 criteria and correlation with preoperative laboratory findings.

Neurosurgical focus

Tamanini JVG,Dal Fabbro M,de Freitas LLL,Vassallo J,de Souza Queiroz L,Rogerio F

434200 was used in Immunohistochemistry to evaluate clincial and laboratory data from pituitary adenoma patients with functioning and non-functioning PA as classified by WHO's 2017 criteria.

Mon Jun 01 00:00:00 UTC 2020

Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.

Scientific reports

Janjic MM,Prévide RM,Fletcher PA,Sherman A,Smiljanic K,Abebe D,Bjelobaba I,Stojilkovic SS

434200 was used in Immunocytochemistry to suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.

Fri Dec 27 00:00:00 UTC 2019

Mutations involving the SRY-related gene SOX8 are associated with a spectrum of human reproductive anomalies.

Human molecular genetics

Portnoi MF,Dumargne MC,Rojo S,Witchel SF,Duncan AJ,Eozenou C,Bignon-Topalovic J,Yatsenko SA,Rajkovic A,Reyes-Mugica M,Almstrup K,Fusee L,Srivastava Y,Chantot-Bastaraud S,Hyon C,Louis-Sylvestre C,Validire P,de Malleray Pichard C,Ravel C,Christin-Maitre S,Brauner R,Rossetti R,Persani L,Charreau EH,Dain L,Chiauzzi VA,Mazen I,Rouba H,Schluth-Bolard C,MacGowan S,McLean WHI,Patin E,Rajpert-De Meyts E,Jauch R,Achermann JC,Siffroi JP,McElreavey K,Bashamboo A

Published figure using NR5A1 monoclonal antibody (Product # 434200) in Immunohistochemistry

Sun Apr 01 00:00:00 UTC 2018

MAPK pathway control of stem cell proliferation and differentiation in the embryonic pituitary provides insights into the pathogenesis of papillary craniopharyngioma.

Development (Cambridge, England)

Haston S,Pozzi S,Carreno G,Manshaei S,Panousopoulos L,Gonzalez-Meljem JM,Apps JR,Virasami A,Thavaraj S,Gutteridge A,Forshew T,Marais R,Brandner S,Jacques TS,Andoniadou CL,Martinez-Barbera JP

434200 was used in Immunohistochemistry to demonstrate a crucial function of the MAPK pathway in controlling the balance between proliferation and differentiation of Sox2+ cells.

Thu Jun 15 00:00:00 UTC 2017

A genomic atlas of human adrenal and gonad development.

Wellcome open research

Del Valle I,Buonocore F,Duncan AJ,Lin L,Barenco M,Parnaik R,Shah S,Hubank M,Gerrelli D,Achermann JC

Published figure using NR5A1 monoclonal antibody (Product # 434200) in Immunohistochemistry

Fri Apr 07 00:00:00 UTC 2017

Steroid hormone synthesis by the ovarian stroma surrounding epithelial ovarian tumors: a potential mechanism in ovarian tumorigenesis.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Blanco LZ,Kuhn E,Morrison JC,Bahadirli-Talbott A,Smith-Sehdev A,Kurman RJ

434200 was used in Immunohistochemistry to support the view that the stroma surrounding epithelial tumors in the ovary is activated to elaborate steroid hormones which may stimulate further neoplastic growth.

Sat Apr 01 00:00:00 UTC 2017

Oncogene-Induced Senescence in Pituitary Adenomas--an Immunohistochemical Study.

Endocrine pathology

Manojlovic-Gacic E,Skender-Gazibara M,Popovic V,Soldatovic I,Boricic N,Raicevic S,Pekic S,Doknic M,Miljic D,Alafuzoff I,Pontén F,Casar-Borota O

434200 was used in immunohistochemistry - paraffin section to report the expression of senescence-associated lysosomal beta-galactosidase, p16, and p21 in different types of pituitary adenomas

Tue Mar 01 00:00:00 UTC 2016

Steroidogenic factor 1 promotes aggressive growth of castration-resistant prostate cancer cells by stimulating steroid synthesis and cell proliferation.

Endocrinology

Lewis SR,Hedman CJ,Ziegler T,Ricke WA,Jorgensen JS

434200 was used in immunohistochemistry - paraffin section to test if steroidogenic factor is expressed in castration-resistant prostate cancer and determine if it stimulates aberrant steroidogenesis and fuels aggressive growth.

Sat Feb 01 00:00:00 UTC 2014

Steroidogenic factor 1 promotes aggressive growth of castration-resistant prostate cancer cells by stimulating steroid synthesis and cell proliferation.

Endocrinology

Lewis SR,Hedman CJ,Ziegler T,Ricke WA,Jorgensen JS

Sat Feb 01 00:00:00 UTC 2014

Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium.

European journal of cancer (Oxford, England : 1990)

Kuhn E,Ayhan A,Shih IeM,Seidman JD,Kurman RJ

434200 was used in immunohistochemistry to identify the origin of ovarian Brenner tumour

Sun Dec 01 00:00:00 UTC 2013

Elevated levels of the steroidogenic factor 1 are associated with over-expression of CYP19 in an oestrogen-producing testicular Leydig cell tumour.

European journal of endocrinology

Straume AH,Løvås K,Miletic H,Gravdal K,Lønning PE,Knappskog S

434200 was used in immunohistochemistry (paraffin) to study CYP19 over-expression in testicular Leydig cell tumors.

Tue May 01 00:00:00 UTC 2012

Elevated levels of the steroidogenic factor 1 are associated with over-expression of CYP19 in an oestrogen-producing testicular Leydig cell tumour.

European journal of endocrinology

Straume AH,Løvås K,Miletic H,Gravdal K,Lønning PE,Knappskog S

434200 was used in immunohistochemistry (paraffin) to study CYP19 over-expression in testicular Leydig cell tumors.

Tue May 01 00:00:00 UTC 2012

ChIP-on-chip analysis reveals angiopoietin 2 (Ang2, ANGPT2) as a novel target of steroidogenic factor-1 (SF-1, NR5A1) in the human adrenal gland.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ferraz-de-Souza B,Lin L,Shah S,Jina N,Hubank M,Dattani MT,Achermann JC

434200 was used in immunohistochemistry - frozen section to identify Ang2 as a novel target of the nuclear receptor steroidogenic factor-1 in the adrenal gland.

Fri Apr 01 00:00:00 UTC 2011

ChIP-on-chip analysis reveals angiopoietin 2 (Ang2, ANGPT2) as a novel target of steroidogenic factor-1 (SF-1, NR5A1) in the human adrenal gland.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ferraz-de-Souza B,Lin L,Shah S,Jina N,Hubank M,Dattani MT,Achermann JC

434200 was used in immunohistochemistry - frozen section to identify Ang2 as a novel target of the nuclear receptor steroidogenic factor-1 in the adrenal gland.

Fri Apr 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: adrenal 4 binding protein; Adrenal 4 binding protein (AD4BP); Adrenal 4-binding protein; ELP; Embryonal long terminal repeat-binding protein; Embryonal LTR-binding protein; fushi tarazu 1 factor homolog; Fushi tarazu factor homolog 1; Fushi tarazu factor homolog 1 (FTZ1); nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; Nuclear receptor subfamily 5 group A member 1 (NR5A1); nuclear receptor subfamily 5, group A, member 1; SF-1; Steroid hormone receptor Ad4BP; Steroid hydroxylase positive regulator; Steroidogenic factor 1; Steroidogenic factor 1 (SF1); steroidogenic factor 1 nuclear receptor; steroidogenic factor-1; STF-1; STF1

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Gene Aliases: AD4BP; Ad4BP/SF-1; ELP; ELP-3; Ftz-F1; FTZ1; FTZF1; hSF-1; NR5A1; POF7; SF-1; SF1; SPGF8; SRXY3

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UniProt ID: (Human) Q5T6F5, (Mouse) P33242, (Rat) P50569

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Entrez Gene ID: (Human) 2516, (Mouse) 26423, (Rat) 83826

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding phospholipid binding zinc ion binding enzyme binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding lipid binding sequence-specific DNA binding metal ion binding

Process(es)

luteinization transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter male gonad development tissue development multicellular organism aging reproductive process cell differentiation adrenal gland development hormone metabolic process steroid hormone mediated signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter maintenance of protein location in nucleus positive regulation of male gonad development negative regulation of female gonad development transcription initiation from RNA polymerase II promoter cell-cell signaling primary sex determination hormone-mediated signaling pathway intracellular receptor signaling pathway regulation of steroid biosynthetic process female gonad development

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NR5A1 Monoclonal Antibody (N1665)

NR5A1 Antibody (434200) in WB

Product Details

434200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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