Proteintech

NRAS Polyclonal Antibody

NRAS Antibody in Immunocytochemistry (ICC/IF)

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NRAS Antibody (10724-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 10724-1-AP (NRAS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NRAS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

10724-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NRAS Fusion Protein Ag1081 (1-189 aa encoded by BC005219)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTEYKLVVV GAGGVGKSAL TIQLIQNHFV DEYDPTIEDS YRKQVVIDGE TCLLDILDTA GQEEYSAMRD QYMRTGEGFL CVFAINNSKS FADINLYREQ IKRVKDSDDV PMVLVGNKCD LPTRTVDTKQ AHELAKSYGI PFIETSAKTR QGVEDAFYTL VREIRQYRMK KLNSSDDGTQ GCMGLPCVVM (1-189 aa encoded by BC005219)

Target Information

NRAS (GTPase NRas) is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML) and Noonan syndrome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

microRNA-203 functions as a natural Ras inhibitor in hepatocellular carcinoma.

American journal of cancer research

Guo J,Li L,Deng N,Xu Y,Wang G,Luo H,Xu C,Li X

Sun May 14 00:00:00 UTC 2023

microRNA-203 functions as a natural Ras inhibitor in hepatocellular carcinoma.

American journal of cancer research

Guo J,Li L,Deng N,Xu Y,Wang G,Luo H,Xu C,Li X

Sun May 14 00:00:00 UTC 2023

microRNA-203 functions as a natural Ras inhibitor in hepatocellular carcinoma.

American journal of cancer research

Guo J,Li L,Deng N,Xu Y,Wang G,Luo H,Xu C,Li X

Sun May 14 00:00:00 UTC 2023

microRNA-203 functions as a natural Ras inhibitor in hepatocellular carcinoma.

American journal of cancer research

Guo J,Li L,Deng N,Xu Y,Wang G,Luo H,Xu C,Li X

Sun May 14 00:00:00 UTC 2023

Near-Infrared Phototheranostic Iron Pyrite Nanocrystals Simultaneously Induce Dual Cell Death Pathways via Enhanced Fenton Reactions in Triple-Negative Breast Cancer.

ACS nano

Zhao C,Liu Z,Chang CC,Chen YC,Zhang Q,Zhang XD,Andreou C,Pang J,Liu ZX,Wang DY,Kircher MF,Yang J

Tue Mar 14 00:00:00 UTC 2023

Epitranscriptic regulation of HRAS by N⁶-methyladenosine drives tumor progression.

Proceedings of the National Academy of Sciences of the United States of America

Pan Y,Gu Y,Liu T,Zhang Q,Yang F,Duan L,Cheng S,Zhu X,Xi Y,Chang X,Ye Q,Gao S

Tue Apr 04 00:00:00 UTC 2023

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16^INK4a levels.

Nature cell biology

Hao X,Shiromoto Y,Sakurai M,Towers M,Zhang Q,Wu S,Havas A,Wang L,Berger S,Adams PD,Tian B,Nishikura K,Kossenkov AV,Liu P,Zhang R

Mon Aug 01 00:00:00 UTC 2022

Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells.

Nature communications

Igarashi N,Miyata K,Loo TM,Chiba M,Hanyu A,Nishio M,Kawasaki H,Zheng H,Toyokuni S,Kon S,Moriyama K,Fujita Y,Takahashi A

Mon Jul 18 00:00:00 UTC 2022

Argonaute 2 modulates EGFR-RAS signaling to promote mutant HRAS and NRAS-driven malignancies.

PNAS nexus

Siebenaler RF,Chugh S,Waninger JJ,Dommeti VL,Kenum C,Mody M,Gautam A,Patel N,Chu A,Bawa P,Hon J,Smith RD,Carlson H,Cao X,Tesmer JJG,Shankar S,Chinnaiyan AM

Fri Jul 01 00:00:00 UTC 2022

ALG3 Promotes Peritoneal Metastasis of Ovarian Cancer through Increasing Interaction of α1,3-mannosylated uPAR and ADAM8.

Cells

Cui X,Pei X,Wang H,Feng P,Qin H,Liu S,Yan Q,Liu J

Thu Oct 06 00:00:00 UTC 2022

Targeting farnesylation as a novel therapeutic approach in HRAS-mutant rhabdomyosarcoma.

Oncogene

Odeniyide P,Yohe ME,Pollard K,Vaseva AV,Calizo A,Zhang L,Rodriguez FJ,Gross JM,Allen AN,Wan X,Somwar R,Schreck KC,Kessler L,Wang J,Pratilas CA

Sun May 01 00:00:00 UTC 2022

Targeting farnesylation as a novel therapeutic approach in HRAS-mutant rhabdomyosarcoma.

Oncogene

Odeniyide P,Yohe ME,Pollard K,Vaseva AV,Calizo A,Zhang L,Rodriguez FJ,Gross JM,Allen AN,Wan X,Somwar R,Schreck KC,Kessler L,Wang J,Pratilas CA

Sun May 01 00:00:00 UTC 2022

RNA helicase DDX5 enables STAT1 mRNA translation and interferon signalling in hepatitis B virus replicating hepatocytes.

Gut

Sun J,Wu G,Pastor F,Rahman N,Wang WH,Zhang Z,Merle P,Hui L,Salvetti A,Durantel D,Yang D,Andrisani O

Sun May 01 00:00:00 UTC 2022

Identification of NRAS Downstream Genes with CRISPR Activation Screening.

Biology

Tatsumi A,Hirakochi H,Inoue S,Tanaka Y,Furuno H,Ikeda M,Ishibashi S,Taguchi T,Yamamoto K,Onishi I,Sachs Z,Largaespada DA,Kitagawa M,Kurata M

Sun Oct 23 00:00:00 UTC 2022

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Cell chemical biology

Qiu N,Abegg D,Guidi M,Gilmore K,Seeberger PH,Adibekian A

Thu Mar 17 00:00:00 UTC 2022

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Cell chemical biology

Qiu N,Abegg D,Guidi M,Gilmore K,Seeberger PH,Adibekian A

Thu Mar 17 00:00:00 UTC 2022

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Analytical chemistry

Chu H,Zhao Q,Liu J,Yang K,Wang Y,Liu J,Zhang K,Zhao B,He H,Zheng Y,Zhong S,Liang Z,Zhang L,Zhang Y

Tue Jan 18 00:00:00 UTC 2022

Discovery of a New CDK4/6 and PI3K/AKT Multiple Kinase Inhibitor Aminoquinol for the Treatment of Hepatocellular Carcinoma.

Frontiers in pharmacology

Xia ZK,Wang W,Qiu JG,Shi XN,Li HJ,Chen R,Ke KB,Dong C,Zhu Y,Wu SG,Zhang RP,Meng ZR,Zhao H,Gu P,Leung KS,Wong MH,Liu XD,Zhou FM,Zhang JY,Yao YT,Wang SJ,Zhang CY,Qin YR,Lin MC,Jiang BH

Tue Aug 03 00:00:00 UTC 2021

Combinations with Allosteric SHP2 Inhibitor TNO155 to Block Receptor Tyrosine Kinase Signaling.

Clinical cancer research : an official journal of the American Association for Cancer Research

Liu C,Lu H,Wang H,Loo A,Zhang X,Yang G,Kowal C,Delach S,Wang Y,Goldoni S,Hastings WD,Wong K,Gao H,Meyer MJ,Moody SE,LaMarche MJ,Engelman JA,Williams JA,Hammerman PS,Abrams TJ,Mohseni M,Caponigro G,Hao HX

Fri Jan 01 00:00:00 UTC 2021

Comprehensive Analysis of the Tumor Microenvironment and Ferroptosis-Related Genes Predict Prognosis with Ovarian Cancer.

Frontiers in genetics

Li XX,Xiong L,Wen Y,Zhang ZJ

Tue Dec 07 00:00:00 UTC 2021

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Biomaterials

Liu Y,Zheng M,Jiao M,Yan C,Xu S,Du Q,Morsch M,Yin J,Shi B

Wed Sep 01 00:00:00 UTC 2021

Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System.

Cell chemical biology

Röth S,Macartney TJ,Konopacka A,Chan KH,Zhou H,Queisser MA,Sapkota GP

Thu Sep 17 00:00:00 UTC 2020

Immunohistochemical assessment of growth factor signaling molecules: MAPK, Akt, and STAT3 pathways in oral epithelial precursor lesions and squamous cell carcinoma.

Odontology

Tashiro K,Oikawa M,Miki Y,Takahashi T,Kumamoto H

Wed Jan 01 00:00:00 UTC 2020

KRAS regulation by small non-coding RNAs and SNARE proteins.

Nature communications

Che Y,Siprashvili Z,Kovalski JR,Jiang T,Wozniak G,Elcavage L,Khavari PA

Mon Nov 11 00:00:00 UTC 2019

Enhanced anti-tumor effect of liposomal Fasudil on hepatocellular carcinoma in vitro and in vivo.

PloS one

Zhao Y,Zhang Y,Vazirinejad Mehdiabad M,Zhou K,Chen Y,Li L,Guo J,Xu C

Wed Mar 11 00:00:00 UTC 2020

Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies.

Science signaling

Waters AM,Ozkan-Dagliyan I,Vaseva AV,Fer N,Strathern LA,Hobbs GA,Tessier-Cloutier B,Gillette WK,Bagni R,Whiteley GR,Hartley JL,McCormick F,Cox AD,Houghton PJ,Huntsman DG,Philips MR,Der CJ

Tue Sep 26 00:00:00 UTC 2017

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Oncotarget

Guo S,Yang P,Jiang X,Li X,Wang Y,Zhang X,Sun B,Zhang Y,Jia Y

Tue Jan 03 00:00:00 UTC 2017

Genetic and epigenetic silencing of mircoRNA-506-3p enhances COTL1 oncogene expression to foster non-small lung cancer progression.

Oncotarget

Guo S,Yang P,Jiang X,Li X,Wang Y,Zhang X,Sun B,Zhang Y,Jia Y

Tue Jan 03 00:00:00 UTC 2017

ANRIL lncRNA triggers efficient therapeutic efficacy by reprogramming the aberrant INK4-hub in melanoma.

Cancer letters

Xu S,Wang H,Pan H,Shi Y,Li T,Ge S,Jia R,Zhang H,Fan X

Mon Oct 10 00:00:00 UTC 2016

ANRIL lncRNA triggers efficient therapeutic efficacy by reprogramming the aberrant INK4-hub in melanoma.

Cancer letters

Xu S,Wang H,Pan H,Shi Y,Li T,Ge S,Jia R,Zhang H,Fan X

Mon Oct 10 00:00:00 UTC 2016

Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.

Molecular carcinogenesis

Liu W,Ning R,Chen RN,Huang XF,Dai QS,Hu JH,Wang YW,Wu LL,Xiong J,Hu G,Guo QL,Yang J,Wang H

Sun May 01 00:00:00 UTC 2016

Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells.

Molecular carcinogenesis

Liu W,Ning R,Chen RN,Huang XF,Dai QS,Hu JH,Wang YW,Wu LL,Xiong J,Hu G,Guo QL,Yang J,Wang H

Sun May 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: GTPase NRas; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; RAS; RASN; Transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog

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Gene Aliases: ALPS4; AV095280; CMNS; HRAS1; N-ras; NCMS; NRAS; NRAS1; NS6

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UniProt ID: (Human) P01111, (Rat) Q04970

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Entrez Gene ID: (Human) 4893, (Mouse) 18176, (Rat) 24605

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Function(s)

GTP binding protein complex binding nucleotide binding GTPase activity molecular_function protein binding

Process(es)

MAPK cascade stimulatory C-type lectin receptor signaling pathway epidermal growth factor receptor signaling pathway Ras protein signal transduction axon guidance Fc-epsilon receptor signaling pathway ERBB2 signaling pathway leukocyte migration establishment of protein localization to Golgi regulation of protein phosphorylation signal transduction small GTPase mediated signal transduction regulation of gene expression positive regulation of fibroblast proliferation negative regulation of skeletal muscle tissue development regulation of cell cycle biological_process positive regulation of cell proliferation visual learning actin cytoskeleton organization regulation of synaptic transmission, GABAergic positive regulation of Rac protein signal transduction negative regulation of neuron apoptotic process negative regulation of cell differentiation regulation of long-term neuronal synaptic plasticity striated muscle cell differentiation epithelial tube branching involved in lung morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NRAS Polyclonal Antibody

NRAS Antibody (10724-1-AP) in ICC/IF

Product Details

10724-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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