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NRCAM Antibody (PA5-116925) in WB
Western blot analysis of NRCAM in extracts of various cell lines. Samples were incubated with NRCAM Polyclonal antibody (Product # PA5-116925) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116925
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-115 of human NRCAM (Q92823)
Conjugate
Form
Liquid
Concentration
1.51 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901555
Product Specific Information
Positive Samples: SH-SY5Y, Mouse spinal cord, Mouse brain
Immunogen sequence: QMISALEVPL DPKLLEDLVQ PPTITQQSPK DYIIDPRENI VIQCEAKGKP PPSFSWTRNG THFDIDKDPL VTMKPGTGTL IINIMSEGKA E
Target Information
Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hBravo; mBravo; MGC138845; MGC138846; neuron-glia-CAM-related cell adhesion molecule; Neuronal cell adhesion molecule; Neuronal surface protein Bravo; Ng-CAM-related; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM
Gene Aliases: Bravo; C030017F07Rik; C130076O07Rik; KIAA0343; mKIAA0343; NRCAM
UniProt ID: (Human) Q92823, (Mouse) Q810U4
Entrez Gene ID: (Human) 4897, (Mouse) 319504
cell adhesion
axon guidance
central nervous system development
protein localization
regulation of neuron projection development
single organismal cell-cell adhesion
neuronal action potential propagation
retinal ganglion cell axon guidance
heterotypic cell-cell adhesion
clustering of voltage-gated sodium channels
angiogenesis
neuron migration
axonogenesis
axonal fasciculation
synapse assembly
regulation of axon extension
positive regulation of neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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