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NRK Antibody (PA5-100912) in ICC/IF
Immunocytochemistry analysis of NRK in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NRK polyclonal antibody (Product # PA5-100912) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100912
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NRK(Accession Q7Z2Y5), corresponding to amino acid residues K292-I342.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850405
Product Specific Information
Antibody detects endogenous levels of total NRK.
Target Information
The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nck-interacting kinase-like embryo specific kinase; NESK; NIK-like embryo-specific kinase; Nik-related protein kinase
Gene Aliases: AA407323; AA409510; AI225895; AV168563; AW538185; NESK; NRK
UniProt ID: (Human) Q7Z2Y5, (Mouse) Q9R0G8
Entrez Gene ID: (Human) 203447, (Mouse) 27206
activation of JNKK activity
parturition
negative regulation of cell proliferation
regulation of spongiotrophoblast cell proliferation
MAPK cascade
protein phosphorylation
regulation of mitotic cell cycle
phosphorylation
stress-activated protein kinase signaling cascade
actin cytoskeleton reorganization
regulation of apoptotic process
neuron projection morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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