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FIGURE: 1 / 1
NSA2 Antibody (PA5-89854) in WB
Western blot analysis of NSA2 in extracts of various cell lines. Samples were incubated with NSA2 Polyclonal antibody (Product # PA5-89854) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-89854
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human NSA2 (NP_0557011)
Conjugate
Form
Liquid
Concentration
1.59 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805803
Product Specific Information
Immunogen sequence: MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAYL LDREGQSRAK VLSNMIKQKR KEKAGKWEVP LPKVRAQGET EVLKVIRTGK RKKKAWKRMV TKVCFVGDGF TRKPPKYERF IRPMGLRFKK AHVTHPELKA TFCLPILGVK KNPSSPLYTT LGVITKGTVI EVNVSELGLV TQGGKVIWGK YAQVTNNPEN DGCINAVLLV; Positive Samples: HeLa, 293T, Mouse heart, Mouse kidney, Mouse skeletal muscle, Rat heart, Rat kidney, Rat skeletal muscle; Cellular Location: Nucleus, nucleolus
Target Information
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ94393; Hairy cell leukemia protein 1; L-name related protein; L-name-related protein 42; LNR42; NSA2 ribosome biogenesis homolog; Ribosome biogenesis protein NSA2 homolog; TGF beta-inducible nuclear protein; TGF beta-inducible nuclear protein 1; TGF-beta inducible nuclear protein; TGF-beta-inducible nuclear protein 1; YR-29
Gene Aliases: 5730427N09Rik; CDK105; HCL-G1; HCLG1; HUSSY-29; HUSSY29; LNR42; NSA2; Nsa2p; TINP1; Yr29
UniProt ID: (Human) O95478, (Mouse) Q9CR47
Entrez Gene ID: (Human) 10412, (Rat) 171456, (Mouse) 59050
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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