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NSD2 Antibody (A303-093A) in WB
Detection of human NSD2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NSD2 antibody (Product # A303-093A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 6 µg/mg lysate. NSD2 was more efficiently immunoprecipitated by rabbit anti-NSD2 antibody BL3768, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Product Details
A303-093A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 525 and 575 of human Nuclear SET Domain-Containing Protein 2.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone-lysine N-methyltransferase NSD2; IL5 promoter REII region-binding protein; MMSET; multiple myeloma SET domain containing protein type III; Multiple myeloma SET domain-containing protein; nuclear receptor binding SET domain protein 2; Nuclear SET domain-containing protein 2; probable histone-lysine N-methyltransferase NSD2; Protein trithorax-5; trithorax/ash1-related protein 5; Wolf-Hirschhorn syndrome candidate 1 protein
Gene Aliases: KIAA1090; MMSET; NSD2; REIIBP; TRX5; WHS; WHSC1
UniProt ID: (Human) O96028
Entrez Gene ID: (Human) 7468
negative regulation of transcription from RNA polymerase II promoter
membranous septum morphogenesis
atrial septum primum morphogenesis
atrial septum secundum morphogenesis
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
anatomical structure morphogenesis
histone H3-K36 methylation
chromatin modification
histone H4-K20 methylation
positive regulation of isotype switching to IgA isotypes
bone development
regulation of establishment of protein localization
regulation of double-strand break repair via nonhomologous end joining
Performance Guarantee
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