Proteintech

NSD3 Polyclonal Antibody

NSD3 Antibody in Immunocytochemistry (ICC/IF)

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NSD3 Antibody (11345-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11345-1-AP (NSD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NSD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

11345-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NSD3 Fusion Protein Ag1894 (1-300 aa encoded by BC012059)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).

Immunogen sequence: MDFSFSFMQ GIMGNTIQQP PQLIDSANIR QEDAFDNNSD IAEDGGQTPY EATLQQGFQY PATTEDLPPL TNGYPSSISV YETQTKYQSY NQYPNGSANG FGAVRNFSPT DYYHSEIPNT RPHEILEKPS PPQPPPPPSV PQTVIPKKTG SPEIKLKITK TIQNGRELFE SSLCGDLLNE VQASEHTKSK HESRKEKRKK SNKHDSSRSE ERKSHKIPKL EPEEQNRPNE RVDTVSEKPR EEPVLKEEAP VQPILSSVPT TEVSTGVKFQ VGDLVWSKVG TYPWWPCMVS SDPQLEVHTK I (1-300 aa encoded by BC012059)

Target Information

This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of novel class inhibitors of NSD3 methyltransferase showing a unique, bivalent binding mode in the SET domain.

Chemical biology & drug design

Kim S,Hwang I,Kim SH,Chung HW,Ji MJ,Moon S,Park HM,Kong G,Hur W

Fri Sep 01 00:00:00 UTC 2023

NSD3, a member of nuclear receptor-binding SET domain family, is a potential prognostic biomarker for pancreatic cancer.

Cancer medicine

Xiong Q,Zhou Y,Zhang S,Zhang Y,Xu Y,Yang Y,Zhou C,Zeng Z,Han J,Zhu Q

Mon May 01 00:00:00 UTC 2023

Dissecting the Immunological Profiles in NSD3-Amplified LUSC through Integrative Multi-Scale Analyses.

Cancers

Xu D,Liu S,Wu X,Marti TM,Dorn P,Schmid RA,Peng RW,Shu Y

Wed Oct 12 00:00:00 UTC 2022

Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair.

Science advances

Liu L,Lin B,Yin S,Ball LE,Delaney JR,Long DT,Gan W

Fri Dec 09 00:00:00 UTC 2022

NSD3-Induced Methylation of H3K36 Activates NOTCH Signaling to Drive Breast Tumor Initiation and Metastatic Progression.

Cancer research

Jeong GY,Park MK,Choi HJ,An HW,Park YU,Choi HJ,Park J,Kim HY,Son T,Lee H,Min KW,Oh YH,Lee JY,Kong G

Fri Jan 01 00:00:00 UTC 2021

High WHSC1L1 Expression Reduces Survival Rates in Operated Breast Cancer Patients with Decreased CD8+ T Cells: Machine Learning Approach.

Journal of personalized medicine

Kim HS,Min KW,Kim DH,Son BK,Kwon MJ,Hong SM

Mon Jul 05 00:00:00 UTC 2021

Development of mammary hyperplasia, dysplasia, and invasive ductal carcinoma in transgenic mice expressing the 8p11 amplicon oncogene NSD3.

Breast cancer research and treatment

Turner-Ivey B,Smith EL,Rutkovsky AC,Spruill LS,Mills JN,Ethier SP

Sat Jul 01 00:00:00 UTC 2017

Development of mammary hyperplasia, dysplasia, and invasive ductal carcinoma in transgenic mice expressing the 8p11 amplicon oncogene NSD3.

Breast cancer research and treatment

Turner-Ivey B,Smith EL,Rutkovsky AC,Spruill LS,Mills JN,Ethier SP

Sat Jul 01 00:00:00 UTC 2017

WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.

Scientific reports

Saloura V,Vougiouklakis T,Zewde M,Deng X,Kiyotani K,Park JH,Matsuo Y,Lingen M,Suzuki T,Dohmae N,Hamamoto R,Nakamura Y

Thu Jan 19 00:00:00 UTC 2017

WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.

Scientific reports

Saloura V,Vougiouklakis T,Zewde M,Deng X,Kiyotani K,Park JH,Matsuo Y,Lingen M,Suzuki T,Dohmae N,Hamamoto R,Nakamura Y

Thu Jan 19 00:00:00 UTC 2017

WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.

Scientific reports

Saloura V,Vougiouklakis T,Zewde M,Deng X,Kiyotani K,Park JH,Matsuo Y,Lingen M,Suzuki T,Dohmae N,Hamamoto R,Nakamura Y

Thu Jan 19 00:00:00 UTC 2017

WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer.

Scientific reports

Saloura V,Vougiouklakis T,Zewde M,Deng X,Kiyotani K,Park JH,Matsuo Y,Lingen M,Suzuki T,Dohmae N,Hamamoto R,Nakamura Y

Thu Jan 19 00:00:00 UTC 2017

Amplification of WHSC1L1 regulates expression and estrogen-independent activation of ERα in SUM-44 breast cancer cells and is associated with ERα over-expression in breast cancer.

Molecular oncology

Irish JC,Mills JN,Turner-Ivey B,Wilson RC,Guest ST,Rutkovsky A,Dombkowski A,Kappler CS,Hardiman G,Ethier SP

Wed Jun 01 00:00:00 UTC 2016

NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation.

Scientific reports

García-Carpizo V,Sarmentero J,Han B,Graña O,Ruiz-Llorente S,Pisano DG,Serrano M,Brooks HB,Campbell RM,Barrero MJ

Thu Sep 08 00:00:00 UTC 2016

NSD2 contributes to oncogenic RAS-driven transcription in lung cancer cells through long-range epigenetic activation.

Scientific reports

García-Carpizo V,Sarmentero J,Han B,Graña O,Ruiz-Llorente S,Pisano DG,Serrano M,Brooks HB,Campbell RM,Barrero MJ

Thu Sep 08 00:00:00 UTC 2016

WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.

Oncotarget

Saloura V,Vougiouklakis T,Zewde M,Kiyotani K,Park JH,Gao G,Karrison T,Lingen M,Nakamura Y,Hamamoto R

Tue Jul 05 00:00:00 UTC 2016

WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.

Oncotarget

Saloura V,Vougiouklakis T,Zewde M,Kiyotani K,Park JH,Gao G,Karrison T,Lingen M,Nakamura Y,Hamamoto R

Tue Jul 05 00:00:00 UTC 2016

WHSC1L1 drives cell cycle progression through transcriptional regulation of CDC6 and CDK2 in squamous cell carcinoma of the head and neck.

Oncotarget

Saloura V,Vougiouklakis T,Zewde M,Kiyotani K,Park JH,Gao G,Karrison T,Lingen M,Nakamura Y,Hamamoto R

Tue Jul 05 00:00:00 UTC 2016

Identification of druggable cancer driver genes amplified across TCGA datasets.

PloS one

Chen Y,McGee J,Chen X,Doman TN,Gong X,Zhang Y,Hamm N,Ma X,Higgs RE,Bhagwat SV,Buchanan S,Peng SB,Staschke KA,Yadav V,Yue Y,Kouros-Mehr H

Mon Jan 19 00:00:00 UTC 2015

Identification of druggable cancer driver genes amplified across TCGA datasets.

PloS one

Chen Y,McGee J,Chen X,Doman TN,Gong X,Zhang Y,Hamm N,Ma X,Higgs RE,Bhagwat SV,Buchanan S,Peng SB,Staschke KA,Yadav V,Yue Y,Kouros-Mehr H

Mon Jan 19 00:00:00 UTC 2015

The histone methyltransferase Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) is involved in human carcinogenesis.

Genes, chromosomes & cancer

Kang D,Cho HS,Toyokawa G,Kogure M,Yamane Y,Iwai Y,Hayami S,Tsunoda T,Field HI,Matsuda K,Neal DE,Ponder BA,Maehara Y,Nakamura Y,Hamamoto R

Fri Feb 01 00:00:00 UTC 2013

The histone methyltransferase Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) is involved in human carcinogenesis.

Genes, chromosomes & cancer

Kang D,Cho HS,Toyokawa G,Kogure M,Yamane Y,Iwai Y,Hayami S,Tsunoda T,Field HI,Matsuda K,Neal DE,Ponder BA,Maehara Y,Nakamura Y,Hamamoto R

Fri Feb 01 00:00:00 UTC 2013

The histone methyltransferase Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) is involved in human carcinogenesis.

Genes, chromosomes & cancer

Kang D,Cho HS,Toyokawa G,Kogure M,Yamane Y,Iwai Y,Hayami S,Tsunoda T,Field HI,Matsuda K,Neal DE,Ponder BA,Maehara Y,Nakamura Y,Hamamoto R

Fri Feb 01 00:00:00 UTC 2013

The histone methyltransferase Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1) is involved in human carcinogenesis.

Genes, chromosomes & cancer

Kang D,Cho HS,Toyokawa G,Kogure M,Yamane Y,Iwai Y,Hayami S,Tsunoda T,Field HI,Matsuda K,Neal DE,Ponder BA,Maehara Y,Nakamura Y,Hamamoto R

Fri Feb 01 00:00:00 UTC 2013

Transforming properties of 8p11-12 amplified genes in human breast cancer.

Cancer research

Yang ZQ,Liu G,Bollig-Fischer A,Giroux CN,Ethier SP

Mon Nov 01 00:00:00 UTC 2010

Transforming properties of 8p11-12 amplified genes in human breast cancer.

Cancer research

Yang ZQ,Liu G,Bollig-Fischer A,Giroux CN,Ethier SP

Mon Nov 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: DKFZp667H044; FLJ20353; Histone-lysine N-methyltransferase NSD3; MGC126766; MGC142029; nuclear receptor binding SET domain protein 3; Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; WHSC1-like protein 1; Wolf-Hirschhorn syndrome candidate 1-like protein 1; Wolf-Hirschhorn syndrome candidate 1-like protein 1 homolog

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Gene Aliases: 6720429E03; A530023P05; AI528490; DC28; NSD3; pp14328; WHISTLE; WHSC1L1

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UniProt ID: (Human) Q9BZ95, (Mouse) Q6P2L6

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Entrez Gene ID: (Human) 54904, (Rat) 290831, (Mouse) 234135

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Function(s)

protein binding zinc ion binding histone-lysine N-methyltransferase activity molecular_function histone methyltransferase activity (H3-K4 specific) histone methyltransferase activity (H3-K27 specific)

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated histone methylation histone lysine methylation biological_process peptidyl-lysine methylation negative regulation of transcription, DNA-templated histone H3-K4 methylation histone H3-K27 methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NSD3 Polyclonal Antibody

NSD3 Antibody (11345-1-AP) in ICC/IF

Product Details

11345-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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