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Bethyl Laboratories
NSF Polyclonal Antibody
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FIGURE: 1 / 1
NSF Antibody (A305-261A) in IP
Detection of human NSF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSF antibody (Product # A305-261A) (lot 1) used for IP at 6 µg per reaction. NSF was also immunoprecipitated by rabbit anti-NSF antibodies BL20744 and BL20747. For blotting immunoprecipitated NSF, A305-261A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-261A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 500 to 550 of human Vesicle-fusing ATPase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Orangutan
Target Information
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N-ethylmaleimide sensitive factor; N-ethylmaleimide-sensitive factor; N-ethylmaleimide-sensitive factor-like protein; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicle-fusing ATPase; Vesicular-fusion protein NSF
Gene Aliases: NSF; SKD2
UniProt ID: (Human) P46459
Entrez Gene ID: (Human) 4905
positive regulation of receptor recycling
potassium ion transport
intracellular protein transport
exocytosis
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
intra-Golgi vesicle-mediated transport
vesicle-mediated transport
regulation of exocytosis
Golgi to plasma membrane protein transport
plasma membrane fusion
positive regulation of protein catabolic process
COPII vesicle coating
Golgi vesicle docking
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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