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NT5C3A Antibody (MA5-29453) in WB
Western Blot using NT5C3A Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29453) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 39 kDa.
Product Details
MA5-29453
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
009
Immunogen
Recombinant Human NT5C3A/UMPH1 Protein (Met12-Leu297)
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785315
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human NT5C3A/UMPH1.
Target Information
Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-nucleotidase, cytosolic III; 7-methylguanosine nucleotidase; 7-methylguanosine phosphate-specific 5'-nucleotidase; cN-III; Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase 3A; Cytosolic 5'-nucleotidase III; lupin; MGC27337; MGC87109; MGC87828; p36; P5'N-1; Pyrimidine 5'-nucleotidase 1; Uridine 5'-monophosphate hydrolase 1
Gene Aliases: cN-III; HSPC233; hUMP1; NT5C3; NT5C3A; p36; P5'N-1; P5N-1; P5N1; PN-I; POMP; PSN1; UMPH; UMPH1
UniProt ID: (Human) Q9H0P0
Entrez Gene ID: (Human) 51251
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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