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NTCP Antibody (PA5-88724) in WB
Western blot analysis of NTCP in mouse brain. Samples were incubated with NTCP Polyclonal antibody (Product # PA5-88724) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88724
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SLC10A1 (NP_0030401)
Conjugate
Form
Liquid
Concentration
0.68 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805091
Product Specific Information
Immunogen sequence: VAVTVLSAIN VGKSIMFAMT PLLIATSSLM PFIGFLLGYV LSALFCLNGR CRRTVSMETG CQNVQLCSTI LNVAFPPEVI GPLFFFPLLY MIFQLGEGLL LIAIFWCYEK FKTPKDKTKM IYTAATTEET IPGALGNGTY KGEDCSPCTA; Positive Samples: Mouse brain; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bile acid cotransporting polypeptide; Hepatic sodium/bile acid cotransporter; Na(+)/bile acid cotransporter; Na(+)/taurocholate transport protein; NA-dependent cholate transporting protein; NTCP; SLC10A1; sodium bile acid cotransporting polypeptide; sodium-dependent bile acid cotransporter; sodium-dependent taurocholate cotransporting polypeptide; sodium-taurocholate cotransporting polypeptide; sodium/bile acid cotransporter; sodium/tauro; Sodium/taurocholate cotransporting polypeptide; solute carrier family 10 (sodium/bile acid cotransporter family), member 1; Solute carrier family 10 member 1; solute carrier family 10, member 1
Gene Aliases: Ntcp; Ntcp1; SBACT; Slc10a1
UniProt ID: (Mouse) O08705, (Rat) P26435
Entrez Gene ID: (Mouse) 20493, (Rat) 24777
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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