Invitrogen

NUDT3 Polyclonal Antibody

NUDT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NUDT3 Antibody (PA5-30435) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NUDT3 (Product # PA5-30435) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

NUDT3 Antibody — Nudt3 down-regulation increases expression of mRNAs implicated in cell motility. (A) RNA levels of nine mRNAs implicated in cell migration tested in MCF-7 cells expressing nontargeting shRNA (ConKD, open bar), shRNA against Nudt3 (Nudt3KD, filled bar), or shRNA against Nudt4 (Nudt4KD, gray bar) are shown. (B) Western blot analysis of Nudt3 protein levels in ConKD or Nudt3KD MCF-7 cells ectopically expressing GFP, Flag-tagged wild-type Nudt3 (Nudt3), or mutant Nudt3 (Nudt3EE/QQ). The band for Flag-Nudt3EE/QQ runs slightly faster than that for Flag-Nudt3 likely due to differences in protein charge by the introduction of glutamic acid residues into the mutant protein. (C) A subset of the mRNAs implicated in cellular movement derived from the 144 mRNAs detected to increase greater than or equal to twofold by RNA-seq analysis in Nudt3KD are shown. (D) Transcript levels of the indicated mRNAs were determined by qRT-PCR in ConKD or Nudt3KD cells complemented with the indicated proteins. RNA levels in A and D are derived from at least three independent experiments and normalized to 18S rRNA and presented relative to the value in control cells set to one. Error bars represent ±SD. P-values are denoted by asterisks. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 (Student's t-test). {KD}

Product Details

PA5-30435

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NUDT3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2547909

Product Specific Information

Recommended positive controls: IMR32.

Predicted reactivity: Mouse (97%), Rat (96%), Dog (99%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

NUDT3 belongs to the MutT, or Nudix, protein family. Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways, guarding against elevated levels of potentially dangerous intermediates, like 8-oxo-dGTP, which promotes AT-to-CG transversions (Safrany et al., 1998 ).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP₇.

Biomolecules

Sahu S,Gordon J,Gu C,Sobhany M,Fiedler D,Stanley RE,Shears SB

PA5-30435 was used in Immunohistochemistry (Paraffin), Western Blot to suggest that 5-InsP7 acts as an 'electrostatic glue' that binds together positively charged surfaces on separate proteins, overcoming mutual protein-protein electrostatic repulsion the latter phenomenon is a known requirement for the assembly of a non-membranous biomolecular condensate.

Thu Jan 12 00:00:00 UTC 2023

Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP₇.

Biomolecules

Sahu S,Gordon J,Gu C,Sobhany M,Fiedler D,Stanley RE,Shears SB

Thu Jan 12 00:00:00 UTC 2023

Nudt3 is an mRNA decapping enzyme that modulates cell migration.

RNA (New York, N.Y.)

Grudzien-Nogalska E,Jiao X,Song MG,Hart RP,Kiledjian M

PA530435 was used in western blot to identify Nudt3 as a Nudix protein with mRNA decapping activity and examine its effects on breast cancer cell migration

Sun May 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Ap6A hydrolase; diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 1; Diadenosine hexaphosphate hydrolase; Diphosphoinositol polyphosphate phosphohydrolase 1; DIPP-1; Endopolyphosphatase; m7GpppN-mRNA hydrolase; m7GpppX diphosphatase; Nucleoside diphosphate-linked moiety X motif 3; nudix (nucleoside diphosphate linked moiety X)-type motif 3; Nudix motif 3

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Gene Aliases: DIPP; DIPP-1; DIPP1; NUDT3

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UniProt ID: (Human) O95989

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Entrez Gene ID: (Human) 11165

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Function(s)

magnesium ion binding diphosphoinositol-polyphosphate diphosphatase activity inositol diphosphate tetrakisphosphate diphosphatase activity inositol bisdiphosphate tetrakisphosphate diphosphatase activity inositol diphosphate pentakisphosphate diphosphatase activity inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity

Process(es)

cell-cell signaling diadenosine polyphosphate catabolic process inositol phosphate metabolic process diphosphoinositol polyphosphate catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NUDT3 Polyclonal Antibody

NUDT3 Antibody (PA5-30435) in IHC (P)

Product Details

PA5-30435

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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