Proteintech

NUFIP1 Polyclonal Antibody

NUFIP1 Antibody in Immunocytochemistry (ICC/IF)

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NUFIP1 Antibody (12515-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 12515-1-AP (NUFIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NUFIP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NUFIP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12515-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NUFIP1 Fusion Protein Ag3197 (1-276 aa encoded by BC017745)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.56 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MHAPGMKKI KLDTPEEIAR WREERRKNYP TLANIERKKK LKLEKEKRGA VLTTTQYGKM KGMSRHSQMA KIRSPGKNHK WKNDNSRQRA VTGSGSHLCD LKLEGPPEAN ADPLGVLINS DSESDKEEKP QHSVIPKEVT PALCSLMSSY GSLSGSESEP EETPIKTEAD VLAENQVLDS SAPKSPSQDV KATVRNFSEA KSENRKKSFE KTNPKRKKDY HNYQTLFEPR THHPYLLEML LAPDIRHERN VILQCVRYII KKDFFGLDTN SAKSKDV (1-276 aa encoded by BC017745)

Target Information

This gene encodes a nuclear RNA binding protein that contains a C2H2 zinc finger motif and a nuclear localization signal. This protein is associated with the nuclear matrix in perichromatin fibrils and, in neurons, localizes to the cytoplasm in association with endoplasmic reticulum ribosomes. This protein interacts with the fragile X mental retardation protein (FMRP), the tumor suppressor protein BRCA1, upregulates RNA polymerase II transcription, and is involved in box C/D snoRNP biogenesis. A pseudogene of this gene resides on chromosome 6q12.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins.

Nature communications

Louche A,Blanco A,Lacerda TLS,Cancade-Veyre L,Lionnet C,Bergé C,Rolando M,Lembo F,Borg JP,Buchrieser C,Nagahama M,Gérard FCA,Gorvel JP,Gueguen-Chaignon V,Terradot L,Salcedo SP

Fri Jan 06 00:00:00 UTC 2023

Nuclear fragile X mental retardation-interacting protein 1-mediated ribophagy protects T lymphocytes against apoptosis in sepsis.

Burns & trauma

Zhao PY,Yao RQ,Zheng LY,Wu Y,Li YX,Dong N,Li JY,Du XH,Yao YM

Tue Mar 07 00:00:00 UTC 2023

Nuclear fragile X mental retardation-interacting protein 1-mediated ribophagy protects T lymphocytes against apoptosis in sepsis.

Burns & trauma

Zhao PY,Yao RQ,Zheng LY,Wu Y,Li YX,Dong N,Li JY,Du XH,Yao YM

Tue Mar 07 00:00:00 UTC 2023

Nuclear fragile X mental retardation-interacting protein 1-mediated ribophagy protects T lymphocytes against apoptosis in sepsis.

Burns & trauma

Zhao PY,Yao RQ,Zheng LY,Wu Y,Li YX,Dong N,Li JY,Du XH,Yao YM

Tue Mar 07 00:00:00 UTC 2023

Nuclear fragile X mental retardation-interacting protein 1-mediated ribophagy protects T lymphocytes against apoptosis in sepsis.

Burns & trauma

Zhao PY,Yao RQ,Zheng LY,Wu Y,Li YX,Dong N,Li JY,Du XH,Yao YM

Tue Mar 07 00:00:00 UTC 2023

The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.

Science advances

Fuentes P,Pelletier J,Martinez-Herráez C,Diez-Obrero V,Iannizzotto F,Rubio T,Garcia-Cajide M,Menoyo S,Moreno V,Salazar R,Tauler A,Gentilella A

Fri Nov 26 00:00:00 UTC 2021

Reduced rDNA transcription diminishes skeletal muscle ribosomal capacity and protein synthesis in cancer cachexia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kim HG,Huot JR,Pin F,Guo B,Bonetto A,Nader GA

Mon Feb 01 00:00:00 UTC 2021

The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.

Autophagy

Shim MS,Nettesheim A,Hirt J,Liton PB

Wed Jul 01 00:00:00 UTC 2020

The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.

Autophagy

Shim MS,Nettesheim A,Hirt J,Liton PB

Wed Jul 01 00:00:00 UTC 2020

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Nature

An H,Ordureau A,Körner M,Paulo JA,Harper JW

Wed Jul 01 00:00:00 UTC 2020

NUFIP1 is a ribosome receptor for starvation-induced ribophagy.

Science (New York, N.Y.)

Wyant GA,Abu-Remaileh M,Frenkel EM,Laqtom NN,Dharamdasani V,Lewis CA,Chan SH,Heinze I,Ori A,Sabatini DM

Fri May 18 00:00:00 UTC 2018

NUFIP1 is a ribosome receptor for starvation-induced ribophagy.

Science (New York, N.Y.)

Wyant GA,Abu-Remaileh M,Frenkel EM,Laqtom NN,Dharamdasani V,Lewis CA,Chan SH,Heinze I,Ori A,Sabatini DM

Fri May 18 00:00:00 UTC 2018

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Nucleic acids research

Gribling-Burrer AS,Leichter M,Wurth L,Huttin A,Schlotter F,Troffer-Charlier N,Cura V,Barkats M,Cavarelli J,Massenet S,Allmang C

Fri May 19 00:00:00 UTC 2017

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Nucleic acids research

Gribling-Burrer AS,Leichter M,Wurth L,Huttin A,Schlotter F,Troffer-Charlier N,Cura V,Barkats M,Cavarelli J,Massenet S,Allmang C

Fri May 19 00:00:00 UTC 2017

NUFIP and the HSP90/R2TP chaperone bind the SMN complex and facilitate assembly of U4-specific proteins.

Nucleic acids research

Bizarro J,Dodré M,Huttin A,Charpentier B,Schlotter F,Branlant C,Verheggen C,Massenet S,Bertrand E

Thu Oct 15 00:00:00 UTC 2015

Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.

Nucleic acids research

Rothé B,Saliou JM,Quinternet M,Back R,Tiotiu D,Jacquemin C,Loegler C,Schlotter F,Peña V,Eckert K,Moréra S,Dorsselaer AV,Branlant C,Massenet S,Sanglier-Cianférani S,Manival X,Charpentier B

Tue Jan 20 00:00:00 UTC 2015

Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.

Nucleic acids research

Rothé B,Saliou JM,Quinternet M,Back R,Tiotiu D,Jacquemin C,Loegler C,Schlotter F,Peña V,Eckert K,Moréra S,Dorsselaer AV,Branlant C,Massenet S,Sanglier-Cianférani S,Manival X,Charpentier B

Tue Jan 20 00:00:00 UTC 2015

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

The Journal of cell biology

Bizarro J,Charron C,Boulon S,Westman B,Pradet-Balade B,Vandermoere F,Chagot ME,Hallais M,Ahmad Y,Leonhardt H,Lamond A,Manival X,Branlant C,Charpentier B,Verheggen C,Bertrand E

Mon Nov 24 00:00:00 UTC 2014

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

The Journal of cell biology

Bizarro J,Charron C,Boulon S,Westman B,Pradet-Balade B,Vandermoere F,Chagot ME,Hallais M,Ahmad Y,Leonhardt H,Lamond A,Manival X,Branlant C,Charpentier B,Verheggen C,Bertrand E

Mon Nov 24 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: FMR1-interacting protein NUFIP1; Nuclear FMR1-interacting protein 1; Nuclear FMRP-interacting protein 1; nuclear fragile X mental retardation protein interacting protein 1

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Gene Aliases: bA540M5.1; NUFIP; NUFIP1

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UniProt ID: (Human) Q9UHK0

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Entrez Gene ID: (Human) 26747

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Function(s)

DNA binding RNA binding protein binding snoRNA binding protein binding, bridging identical protein binding metal ion binding ATPase binding

Process(es)

box C/D snoRNP assembly RNA processing positive regulation of transcription from RNA polymerase II promoter protein oligomerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NUFIP1 Polyclonal Antibody

NUFIP1 Antibody (12515-1-AP) in ICC/IF

Product Details

12515-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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