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Invitrogen
NUMB Polyclonal Antibody
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FIGURE: 1 / 5
NUMB Antibody (PA1-31840) in ICC/IF
Immunofluorescence analysis of NUMB was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NUMB Polyclonal Antibody (Product # PA1-31840) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a dilut... View More
Product Details
PA1-31840
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2154284
Product Specific Information
Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX24147-PEP)..
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: h-Numb; m-Nb; m-numb; mnb; numb gene homolog; numb homolog; Protein numb homolog; Protein S171
Gene Aliases: c14_5527; C14orf41; Nb; NUMB; S171
UniProt ID: (Human) P49757, (Mouse) Q9QZS3
Entrez Gene ID: (Human) 8650, (Mouse) 18222, (Rat) 29419
multicellular organism development
nervous system development
neuroblast proliferation
axonogenesis
lateral ventricle development
neuroblast division in subventricular zone
positive regulation of cell migration
positive regulation of polarized epithelial cell differentiation
forebrain development
adherens junction organization
regulation of neuron differentiation
negative regulation of Notch signaling pathway
positive regulation of neurogenesis
positive regulation of dendrite morphogenesis
lung epithelial cell differentiation
negative regulation of protein localization to plasma membrane
cell-cell adhesion
cell proliferation
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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