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NUP214 Antibody (PA5-114371) in ICC/IF
Immunocytochemistry analysis of NUP214 using anti-NUP214 antibody (Product # PA5-114371). NUP214 was detected in a section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NUP214 antibody (Product # PA5-114371) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with... View More
Product Details
PA5-114371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NUP214 recombinant protein (Position: K36-E374).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884827
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human K562 whole cell, human THP-1 whole cell, mouse spleen tissue, mouse thymus tissue. ICC/IF: A431 cell, NIH/3T3 cell. Flow: HL-60 cell, HEPA1-6 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 214 kDa nucleoporin; CAN protein, putative oncogene; Nuclear pore complex protein Nup214; nucleoporin 214kDa; Nucleoporin Nup214; Protein CAN
Gene Aliases: BC039282; CAIN; CAN; D2H9S46E; KIAA0023; NUP214
UniProt ID: (Human) P35658, (Mouse) Q80U93
Entrez Gene ID: (Human) 8021, (Mouse) 227720
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
protein export from nucleus
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
regulation of mRNA stability
regulation of cell cycle
intracellular transport of virus
regulation of cellular response to heat
transport
protein transport
mRNA transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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