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NUP43 Antibody (A303-975A) in IP
Detection of human NUP43 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUP43 antibody (Product # A303-975A) (lot 1) used for IP at 6 µg/mg lysate. NUP43 was inefficiently immunoprecipitated by rabbit anti-NUP43 antibody A303-976A. For blotting immunoprecipitated NUP43, A303-976A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-975A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 300 to 350 of human Nucleoporin 43kDa.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: nucleoporin 43kDa; Nucleoporin Nup43; Nup107-160 subcomplex subunit Nup43; p42
Gene Aliases: bA350J20.1; NUP43; p42
UniProt ID: (Human) Q8NFH3
Entrez Gene ID: (Human) 348995
mRNA export from nucleus
tRNA export from nucleus
sister chromatid cohesion
mitotic nuclear division
mitotic nuclear envelope disassembly
regulation of glucose transport
protein transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
cell division
intracellular transport of virus
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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