Proteintech

NUR77 Polyclonal Antibody

NUR77 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

NUR77 Antibody (12235-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 12235-1-AP (NUR77 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

12235-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 7 publications 7 publications

ELISA (ELISA)

View 1 publication 1 publication

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NUR77 Fusion Protein Ag2875 (299-598 aa encoded by BC016147)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG RLPSKPKQPP DASPANLLTS LVRAHLDSGP STAKLDYSKF QELVLPHFGK EDAGDVQQFY DLLSGSLEVI RKWAEKIPGF AELSPADQDL LLESAFLELF ILRLAYRSKP GEGKLIFCSG LVLHRLQCAR GFGDWIDSIL AFSRSLHSLL VDVPAFACLS ALVLITDRHG LQEPRRVEEL QNRIASCLKE HVAAVAGEPQ PASCLSRLLG KLPELRTLCT QGLQRIFYLK LEDLVPPPPI IDKIFMDTLP F (299-598 aa encoded by BC016147)

Target Information

NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. NGFI-B-alpha is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. The encoded protein acts as a nuclear transcription factor involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Three transcript variants encoding two distinct isoforms have been identified for this NR4A1. Variant (3), also known as TRC beta, contains multiple differences compared to variant 1. The resulting isoform b contains a shorter and distinct C-terminus compared to isoform a. RXR has been shown to be a partner for NR4A1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hederagenin suppresses glioma cell biological activities via Nur77 in vitro study.

Food science & nutrition

Dai Y,Masra N,Zhou L,Yu C,Jin W,Ni H

Wed Mar 01 00:00:00 UTC 2023

Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.

Cell death discovery

Sheng M,Weng Y,Cao Y,Zhang C,Lin Y,Yu W

Tue Mar 28 00:00:00 UTC 2023

β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals.

Immunity, inflammation and disease

Zhu F,Zhang Q,Feng J,Zhang X,Li T,Liu S,Chen Y,Li X,Wu Q,Xue Y,Alitongbieke G,Pan Y

Mon May 01 00:00:00 UTC 2023

β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals.

Immunity, inflammation and disease

Zhu F,Zhang Q,Feng J,Zhang X,Li T,Liu S,Chen Y,Li X,Wu Q,Xue Y,Alitongbieke G,Pan Y

Mon May 01 00:00:00 UTC 2023

β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals.

Immunity, inflammation and disease

Zhu F,Zhang Q,Feng J,Zhang X,Li T,Liu S,Chen Y,Li X,Wu Q,Xue Y,Alitongbieke G,Pan Y

Mon May 01 00:00:00 UTC 2023

β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals.

Immunity, inflammation and disease

Zhu F,Zhang Q,Feng J,Zhang X,Li T,Liu S,Chen Y,Li X,Wu Q,Xue Y,Alitongbieke G,Pan Y

Mon May 01 00:00:00 UTC 2023

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Nature metabolism

Chen QT,Zhang ZY,Huang QL,Chen HZ,Hong WB,Lin T,Zhao WX,Wang XM,Ju CY,Wu LZ,Huang YY,Hou PP,Wang WJ,Zhou D,Deng X,Wu Q

Sat Oct 01 00:00:00 UTC 2022

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Nature metabolism

Chen QT,Zhang ZY,Huang QL,Chen HZ,Hong WB,Lin T,Zhao WX,Wang XM,Ju CY,Wu LZ,Huang YY,Hou PP,Wang WJ,Zhou D,Deng X,Wu Q

Sat Oct 01 00:00:00 UTC 2022

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Nature metabolism

Chen QT,Zhang ZY,Huang QL,Chen HZ,Hong WB,Lin T,Zhao WX,Wang XM,Ju CY,Wu LZ,Huang YY,Hou PP,Wang WJ,Zhou D,Deng X,Wu Q

Sat Oct 01 00:00:00 UTC 2022

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Nature metabolism

Chen QT,Zhang ZY,Huang QL,Chen HZ,Hong WB,Lin T,Zhao WX,Wang XM,Ju CY,Wu LZ,Huang YY,Hou PP,Wang WJ,Zhou D,Deng X,Wu Q

Sat Oct 01 00:00:00 UTC 2022

hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms.

Reproductive biology and endocrinology : RB&E

Luo Q,Tang Y,Jiang Z,Bao H,Fu Q,Zhang H

Fri Aug 19 00:00:00 UTC 2022

hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms.

Reproductive biology and endocrinology : RB&E

Luo Q,Tang Y,Jiang Z,Bao H,Fu Q,Zhang H

Fri Aug 19 00:00:00 UTC 2022

hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms.

Reproductive biology and endocrinology : RB&E

Luo Q,Tang Y,Jiang Z,Bao H,Fu Q,Zhang H

Fri Aug 19 00:00:00 UTC 2022

The epilepsy-associated protein PCDH19 undergoes NMDA receptor-dependent proteolytic cleavage and regulates the expression of immediate-early genes.

Cell reports

Gerosa L,Mazzoleni S,Rusconi F,Longaretti A,Lewerissa E,Pelucchi S,Murru L,Giannelli SG,Broccoli V,Marcello E,Kasri NN,Battaglioli E,Passafaro M,Bassani S

Tue May 24 00:00:00 UTC 2022

TIAM1-RAC1 promote small-cell lung cancer cell survival through antagonizing Nur77-induced BCL2 conformational change.

Cell reports

Payapilly A,Guilbert R,Descamps T,White G,Magee P,Zhou C,Kerr A,Simpson KL,Blackhall F,Dive C,Malliri A

Tue Nov 09 00:00:00 UTC 2021

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution.

Aging

Gong Y,Yang J,Li X,Zhou C,Chen Y,Wang Z,Qiu X,Liu Y,Zhang H,Greenbaum J,Cheng L,Hu Y,Xie J,Yang X,Li Y,Bai Y,Wang YP,Chen Y,Tan LJ,Shen H,Xiao HM,Deng HW

Tue Aug 24 00:00:00 UTC 2021

Simvastatin Improves Behavioral Disorders and Hippocampal Inflammatory Reaction by NMDA-Mediated Anti-inflammatory Function in MPTP-Treated Mice.

Cellular and molecular neurobiology

Yan J,Liu A,Fan H,Qiao L,Wu J,Shen M,Lai X,Huang J

Thu Oct 01 00:00:00 UTC 2020

Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.

Endocrinology

Kameda H,Yamamoto M,Tone Y,Tone M,Melmed S

Fri Feb 01 00:00:00 UTC 2019

Ovarian tumor domain-containing ubiquitin aldehyde binding protein 1 inhibits inflammation by regulating Nur77 stability.

Cellular signalling

Pei HZ,Huang B,Chang HW,Baek SH

Mon Jul 01 00:00:00 UTC 2019

The E3 ubiquitin ligase Trim13 regulates Nur77 stability via casein kinase 2α.

Scientific reports

Huang B,Pei HZ,Chang HW,Baek SH

Mon Sep 17 00:00:00 UTC 2018

The E3 ubiquitin ligase Trim13 regulates Nur77 stability via casein kinase 2α.

Scientific reports

Huang B,Pei HZ,Chang HW,Baek SH

Mon Sep 17 00:00:00 UTC 2018

Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.

Molecular neurobiology

Wei X,Gao H,Zou J,Liu X,Chen D,Liao J,Xu Y,Ma L,Tang B,Zhang Z,Cai X,Jin K,Xia Y,Wang Q

Tue Nov 01 00:00:00 UTC 2016

Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.

Molecular neurobiology

Wei X,Gao H,Zou J,Liu X,Chen D,Liao J,Xu Y,Ma L,Tang B,Zhang Z,Cai X,Jin K,Xia Y,Wang Q

Tue Nov 01 00:00:00 UTC 2016

Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.

Molecular neurobiology

Wei X,Gao H,Zou J,Liu X,Chen D,Liao J,Xu Y,Ma L,Tang B,Zhang Z,Cai X,Jin K,Xia Y,Wang Q

Tue Nov 01 00:00:00 UTC 2016

Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.

Molecular neurobiology

Wei X,Gao H,Zou J,Liu X,Chen D,Liao J,Xu Y,Ma L,Tang B,Zhang Z,Cai X,Jin K,Xia Y,Wang Q

Tue Nov 01 00:00:00 UTC 2016

Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.

Scientific reports

Gao H,Chen Z,Fu Y,Yang X,Weng R,Wang R,Lu J,Pan M,Jin K,McElroy C,Tang B,Xia Y,Wang Q

Thu Sep 29 00:00:00 UTC 2016

Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.

Scientific reports

Gao H,Chen Z,Fu Y,Yang X,Weng R,Wang R,Lu J,Pan M,Jin K,McElroy C,Tang B,Xia Y,Wang Q

Thu Sep 29 00:00:00 UTC 2016

NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.

Scientific reports

Zhang Y,Chen G,Gao B,Li Y,Liang S,Wang X,Wang X,Zhu B

Wed Nov 23 00:00:00 UTC 2016

NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.

Scientific reports

Zhang Y,Chen G,Gao B,Li Y,Liang S,Wang X,Wang X,Zhu B

Wed Nov 23 00:00:00 UTC 2016

NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.

Scientific reports

Zhang Y,Chen G,Gao B,Li Y,Liang S,Wang X,Wang X,Zhu B

Wed Nov 23 00:00:00 UTC 2016

NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.

Scientific reports

Zhang Y,Chen G,Gao B,Li Y,Liang S,Wang X,Wang X,Zhu B

Wed Nov 23 00:00:00 UTC 2016

Antitumor activity of SAHA, a novel histone deacetylase inhibitor, against murine B cell lymphoma A20 cells in vitro and in vivo.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Yang B,Yu D,Liu J,Yang K,Wu G,Liu H

Wed Jul 01 00:00:00 UTC 2015

The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.

The Journal of biological chemistry

Yu C,Cui S,Zong C,Gao W,Xu T,Gao P,Chen J,Qin D,Guan Q,Liu Y,Fu Y,Li X,Wang X

Fri Aug 21 00:00:00 UTC 2015

The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.

The Journal of biological chemistry

Yu C,Cui S,Zong C,Gao W,Xu T,Gao P,Chen J,Qin D,Guan Q,Liu Y,Fu Y,Li X,Wang X

Fri Aug 21 00:00:00 UTC 2015

The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.

The Journal of biological chemistry

Yu C,Cui S,Zong C,Gao W,Xu T,Gao P,Chen J,Qin D,Guan Q,Liu Y,Fu Y,Li X,Wang X

Fri Aug 21 00:00:00 UTC 2015

The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.

The Journal of biological chemistry

Yu C,Cui S,Zong C,Gao W,Xu T,Gao P,Chen J,Qin D,Guan Q,Liu Y,Fu Y,Li X,Wang X

Fri Aug 21 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; immediate early gene transcription factor NGFI-B; MGC9485; nerve growth factor IB nuclear receptor variant 1; nerve growth factor induced protein I-B; Nerve growth factor-induced protein I-B; NGFI-B; Nuclear hormone receptor NUR/77; Nuclear protein N10; Nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nuclear receptor subfamily 4immunitygroup A member 1; NUR77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; Orphan receptor tr3; ST-59; steroid receptor TR3; Testicular receptor 3; TR3 orphan receptor

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Gene Aliases: Gfrp; GFRP1; Hbr-1; Hbr1; HMR; N10; NAK-1; NAK1; NGFI-B; NGFIB; NP10; NR4A1; NUR77; TIS1; TR3

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UniProt ID: (Human) P22736, (Rat) P22829, (Mouse) P12813

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Entrez Gene ID: (Human) 3164, (Rat) 79240, (Mouse) 15370

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Function(s)

transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding protein heterodimerization activity protein homodimerization activity transcription factor activity, sequence-specific DNA binding metal ion binding

Process(es)

positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis transcription, DNA-templated transcription from RNA polymerase II promoter apoptotic process intracellular receptor signaling pathway endothelial cell chemotaxis skeletal muscle cell differentiation cellular response to vascular endothelial growth factor stimulus positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process steroid hormone mediated signaling pathway cellular response to fibroblast growth factor stimulus positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cellular response to organic substance transcription initiation from RNA polymerase II promoter signal transduction fat cell differentiation negative regulation of cell cycle regulation of type B pancreatic cell proliferation cellular response to corticotropin-releasing hormone stimulus regulation of transcription, DNA-templated response to inorganic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NUR77 Polyclonal Antibody

NUR77 Antibody (12235-1-AP) in ICC/IF

Product Details

12235-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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