GenScript

NWSHPQFEK Tag Monoclonal Antibody (5A9F9), FITC, THE™

NWSHPQFEK Tag Antibody in Flow Cytometry (Flow)

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NWSHPQFEK Tag Antibody (A01736-100) in Flow

Flow cytometric analysis of non-transfected CHO cells (a) or Strep II fusion protein transfected CHO cells (b) using THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse (Cat. No. A01736).

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Product Details

A01736-100

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-3 µg/mL

Flow Cytometry (Flow)

1-3 µg 1 x 10^6 cells

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5A9F9

Immunogen

NWSHPQFEK peptide conjugated to KLH

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. Strep II tag (NWSHPQFEK) is a nine amino acid peptide with high specificity and affinity towards streptavidin. GenScript THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with FITC. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.

Target Information

The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

A novel luciferase-based assay for the detection of Chimeric Antigen Receptors.

Scientific reports

Gopalakrishnan R,Matta H,Choi S,Natarajan V,Prins R,Gong S,Zenunovic A,Narasappa N,Patel F,Prakash R,Chaudhary V,Sikri V,Chitnis SD,Kochegarov A,Wang D,Falat M,Kahn M,Keerthipati PS,Sharma N,Lenka J,Stieben TM,Braun J,Batra A,Purvis K,Ito K,Lee JH,Jeronimo A,Zamora HM,Membreno A,Qiu Q,Peshin S,Namburu L,Chaudhary PM

Chimeric Antigen Receptor-T (CAR-T) cell immunotherapy has produced dramatic responses in hematologic malignancies. One of the challenges in the field is the lack of a simple assay for the detection of CARs on the surface of immune effector cells. In this study we describe a novel luciferase-based assay termed Topanga Assay for the detection of CAR expression. The assay utilizes a recombinant fusion protein called Topanga reagent generated by joining the extra-cellular domain of a CAR-target in

Wed Feb 13 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Streptavidin; Streptomyces

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