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GenScript
NWSHPQFEK Tag Monoclonal Antibody (5A9F9), Biotin, THE™
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NWSHPQFEK Tag Antibody (A01737-100) in WB
Western blot analysis of N-terminal and C-terminal Strep II tagged fusion protein using THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse (GenScript, A01737, 1 µg/mL). The signal was developed with Streptaidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor THE™ HRP Substrate Kit (GenScript, L00221).
Product Details
A01737-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5A9F9
Immunogen
NWSHPQFEK peptide conjugated to KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 10mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.GenScript THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
Target Information
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. The original Strep-tag was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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