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NXPH4 Antibody (PA5-101711) in ICC/IF
Immunofluorescent analysis of NXPH4 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and NXPH4 polyclonal antibody (Product # PA5-101711) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NXPH4(Accession O95158), corresponding to amino acid residues G218-W268.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851145
Product Specific Information
Antibody detects endogenous levels of total NXPH4.
Target Information
Members of the Neurexophilin family are neuropeptide-like glycoproteins that undergo proteolytic processing after synthesis. As secreted proteins, Neurexophilins are thought to function as signaling molecules which specifically bind to target proteins, such as neurexin I-alpha (a protein that promotes adhesion between dendrites and axons), and are essential for proper neurotransmitter release. Neurexophilins contain five common domains including a variable N-terminal domain, a highly conserved N-glycosylated central domain, a short linker region and a cysteine-rich conserved C-terminal domain. Neurexophilin-4, also known as NXPH4 or NPH4, is a 308 amino acid secreted protein belonging to the neurexophilin family. Neurexophilin-4 is expressed in brain, spleen and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alternative protein NXPH4; Neurexophilin-4
Gene Aliases: 1110036M10Rik; AI851051; NPH4; NXPH4; UNQ1928/PRO4403
UniProt ID: (Human) O95158, (Rat) Q9Z2N4
Entrez Gene ID: (Human) 11247, (Mouse) 104080, (Rat) 59316
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